Characterization of Heterologous and Native Enzyme Activity Profiles in Metabolically Engineered Zymomonas mobilis Strains During Batch Fermentation of Glucose and Xylose Mixtures
| Autor: | Dhinakar S. Kompala, Qiang Gao, Min Zhang, James D. McMillan |
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| Rok vydání: | 2002 |
| Předmět: |
Glycerol
Pentose Bioengineering Acetates Pentose phosphate pathway Xylose Protein Engineering Applied Microbiology and Biotechnology Biochemistry Zymomonas mobilis Metabolic engineering chemistry.chemical_compound Xylose metabolism Lactic Acid Molecular Biology Xylitol chemistry.chemical_classification Zymomonas Ethanol biology General Medicine biology.organism_classification Arabinose Kinetics Metabolic pathway Glucose Enzyme chemistry Fermentation Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. :341-356 |
| ISSN: | 0273-2289 |
| DOI: | 10.1385/abab:98-100:1-9:341 |
| Popis: | Zymomonas mobilis has been metabolically engineered to broaden its substrate utilization range to include D-xylose and L-arabinose. Both genomically integrated and plasmid-bearing Z. mobilis strains that are capable of fermenting the pentose D-xylose have been created by incorporating four genes: two genes encoding xylose utilization metabolic enzymes (xylA/xylB) and two genes encoding pentose phosphate pathway enzymes (talB/tktA). We have characterized the activities of the four newly introduced enzymes for xylose metabolism, along with those of three native glycolytic enzymes, in two different xylose-fermenting Z. mobilis strains. These strains were grown on glucose-xylose mixtures in computer-controlled fermentors. Samples were collected and analyzed to determine extracellular metabolite concentrations as well as the activities of several intracellular enzymes in the xylose and glucose uptake and catabolism pathways. These measurements provide new insights on the possible bottlenecks in the engineered metabolic pathways and suggest methods for further improving the efficiency of xylose fermentation. |
| Databáze: | OpenAIRE |
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