Human epidermal in vitro permeation test (IVPT) analyses of alcohols and steroids

Autor: Azadeh Alinaghi, Ana Macedo, Hanumanth S. Cheruvu, Amy Holmes, Michael S. Roberts
Přispěvatelé: Alinaghi, Azadeh, Macedo, Ana, Cheruvu, Hanumanth S, Holmes, Amy, Roberts, Michael S
Rok vydání: 2022
Předmět:
Zdroj: International journal of pharmaceutics. 627
ISSN: 1873-3476
Popis: This study examined a number of factors that can impact the outcomes of in vitro human epidermal permeation coefficients for aliphatic alcohols and steroids, including receptor phase composition and study conditions. We determined experimentally the solubilities and IVPT permeation of a homologous series of 14C labeled aliphatic alcohols (ethanol, propanol, pentanol, heptanol, octanol and decanol) in different receptor fluids as recom-mended by Organisation Economic Co-operation and Development (OECD). We used human epidermal mem-branes at 25 degrees C and phosphate-buffered saline (PBS), 2 %w/v bovine serum albumin (2 %w/v BSA), 50 %w/v ethanol and 0.1, 2 and 6 %w/v Oleth-20 receptor phases. We also explored and confirmed the discrepancies between in vitro human epidermal permeability coefficients (kp) and diffusion lag times for steroids from Scheuplein's group with our own work and that of others. The main reason for the observed differences is not clear but is likely to be multifactorial, including the effects of diffusion cell design, receptor phase solubility, unstirred receptor phase effects, epidermal membrane hydration, diffusion cell configuration, transport through appendageal pathways and steroid lipophilicity. We conclude with a summary of experimental conditions that should be considered in undertaking IVPT studies. Refereed/Peer-reviewed
Databáze: OpenAIRE
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