Clinicopathological Features of ALK Expression in 9889 Cases of Non-small-Cell Lung Cancer and Genomic Rearrangements Identified by Capture-Based Next-Generation Sequencing: A Chinese Retrospective Analysis
| Autor: | Min Ye, Gang Qin, Ruiying Zhao, Jinchen Shao, Wenjie Ding, Chan Xiang, Jikai Zhao, Lanxiang Zhao, Yuchen Han, Qing Zhang, Jie Zhang, Lei Zhu, Haohua Teng |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male China Lung Neoplasms Biology DNA sequencing 03 medical and health sciences Young Adult 0302 clinical medicine hemic and lymphatic diseases Carcinoma Non-Small-Cell Lung Genetics medicine Carcinoma Anaplastic lymphoma kinase Humans Anaplastic Lymphoma Kinase Genetic Predisposition to Disease Lung cancer Genetic Association Studies Aged Neoplasm Staging Retrospective Studies Pharmacology Aged 80 and over Gene Rearrangement High-Throughput Nucleotide Sequencing General Medicine Gene rearrangement Middle Aged medicine.disease Molecular medicine Immunohistochemistry 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Molecular Medicine Adenocarcinoma Female |
| Zdroj: | Molecular diagnosistherapy. 23(3) |
| ISSN: | 1179-2000 |
| Popis: | The clinicopathological features and genomic rearrangements of anaplastic lymphoma kinase (ALK) fusion cases have not been fully identified. Our objective was to explore the status of ALK in non-small-cell lung cancer (NSCLC) specimens, to explore the relationships between ALK status and clinicopathological features and to identify genomic rearrangements via capture-based next-generation sequencing (NGS). We tested 9889 NSCLC specimens for ALK status using the Ventana anti-ALK (D5F3) antibody. Clinicopathological features were analyzed and genomic rearrangements identified using capture-based NGS in 76 ALK-positive cases. In total, 485 specimens (4.90%) tested positive for ALK. The positivity rate was higher for adenocarcinoma samples than for non-adenocarcinoma samples (6.03 vs. 1.47%; p |
| Databáze: | OpenAIRE |
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