Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment
| Autor: | Mariyam K. Alshammari, Dunia A. Al Farraj, T. Sujin Jeba Kumar, Noorah A. Alkubaisi, Ponnuswamy Vijayaraghavan, Roua M. Alkufeidy, Mohamed Soliman Elshikh |
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| Rok vydání: | 2020 |
| Předmět: |
Central composite design
Fibrinolytic agent Metal ions in aqueous solution Blood clot chemistry.chemical_element Bacillus Cheap substrates 02 engineering and technology 010501 environmental sciences 01 natural sciences Fibrinolytic enzyme chemistry.chemical_compound Fish meal Food science Response surface methodology lcsh:Science (General) 0105 earth and related environmental sciences chemistry.chemical_classification Multidisciplinary Magnesium Factorial experiment Maltose 021001 nanoscience & nanotechnology Enzyme chemistry 0210 nano-technology lcsh:Q1-390 |
| Zdroj: | Journal of King Saud University: Science, Vol 32, Iss 7, Pp 3174-3180 (2020) |
| ISSN: | 1018-3647 |
| Popis: | Objectives The main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus. Methods Fish meal of Sardinella longiceps and anchovy was optimized using a two-level full factorial design (25) and response surface methodology. The significant physical factors and nutrient sources (peptone, maltose, and magnesium chloride) were identified by statistical approach. The properties of a purified enzyme including their effect at different temperature, pH and the effect of metal ions were evaluated. Results Enzyme yield was improved 3.5 fold than unoptimized medium. Central composite design optimized culture medium enhanced enzyme yield (4711 ± 29.3 U/g of substrate). The fibrinolytic enzyme was highly active at alkaline pH (8.0), 50 °C and the molecular weight was 32 kDa. Conclusions From these findings, it concludes that this fibrinolytic enzyme could be a novel potent thrombolytic agent. |
| Databáze: | OpenAIRE |
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