The fate of paternal mitochondria in marmoset pre-implantation embryos
| Autor: | C.M. Luetjens, Ramona Wesselmann |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Embryonic Development Mitochondrion biology.animal medicine Animals Cells Cultured Loss function Genetics Staining and Labeling General Veterinary biology Cytochromes c Marmoset Callithrix Embryo Embryo Mammalian Oocyte biology.organism_classification Spermatozoa Sperm Mitochondria Cell biology medicine.anatomical_structure Fertilization Oocytes Female Animal Science and Zoology Immunostaining |
| Zdroj: | Journal of Medical Primatology. 37:128-140 |
| ISSN: | 1600-0684 0047-2565 |
| Popis: | Background Sperm-derived mitochondria are integrated into the oocyte at fertilization but seem to vanish during the early cleavage phase. The developmental potential of pre-implantation embryos seems to be closely related to their ability to induce degeneration of these mitochondria, but the mechanisms underlying their loss of function are not yet understood. This study focuses on the fate of paternal mitochondria in pre-implantation embryos. Methods Stimulation, collection and in vitro culture of oocytes from Callithrix jacchus, allows the study of the destiny of paternal mitochondria by utilizing immunostaining of pre-implantation embryos, fluorescence and laserscanning microscopy. Live pre-implantation embryos were stained with a fluorescence indicator reflecting mitochondrial membrane potential. Results Evidence indicating the loss of mitochondrial function was not found nor that apoptosis pathways were involved in the disappearance of paternally derived mitochondria. Conclusions These findings may have implications for mitochondrially inherited diseases and could lead to new strategies for improving assisted reproduction. |
| Databáze: | OpenAIRE |
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