Bioreduction of carboxylic acids by Pyrococcus furiosus in batch cultures
| Autor: | Hans Wassink, Hendra M. Willemen, Colja Laane, Huub Haaker, Eyke C. D. van den Ban |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Hexanoic acid biology Chemistry Aldehyde reduction Substrate specificity Carboxylic acid Biochemie Bioengineering Alcohol Carboxylic acid reduction biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Aldehyde Pyrococcus furiosus chemistry.chemical_compound Hyperthermophilic archaeon Yield (chemistry) Organic chemistry Aliphatic compound Energy source VLAG Biotechnology |
| Zdroj: | Enzyme and Microbial Technology 25 (1999) Enzyme and Microbial Technology, 25, 251-257 |
| ISSN: | 0141-0229 |
| DOI: | 10.1016/s0141-0229(99)00036-8 |
| Popis: | The reduction of aromatic and aliphatic (di)carboxylic acids to their corresponding aldehydes and alcohols by the hyperthermophilic organism Pyrococcus furiosus was investigated. The reduction was performed with P. furiosus cells growing in the presence of 1 mM acid with starch as a carbon and energy source at 90oC. The aromatic acids t-cinnamic and 3-phenylpropionic acid were reduced to their corresponding alcohols with the highest yields in the described batch cultures: 67 and 69%, respectively. The aliphatic acid reduced with the highest yield was hexanoic acid (yield: 38%). No aldehydes were detected during the reduction of acids, indicating that the reduction of aldehydes to alcohols is faster than the reduction of acids to aldehydes. Some aldehydes were both reduced to the corresponding alcohol and oxidized to the corresponding acid. Besides reduction of the unsaturated t-cinnamaldehyde to t-cinnamyl alcohol (63%), the double bond of t-cinnamaldehyde was also reduced by P. furiosus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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