Development of a high-performance liquid chromatography method for the determination of florfenicol in animal feedstuffs
| Autor: | JinJing Yang, GuiZhi Sun, Bo Yang, Lingli Huang, MingRong Qian, XianBing Ke |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Florfenicol
Animal feed Clinical Biochemistry Ethyl acetate 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Limit of Detection medicine Chromatography High Pressure Liquid Thiamphenicol Detection limit Chromatography 010405 organic chemistry Chemistry 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Animal Feed Thin-layer chromatography 0104 chemical sciences Standard curve Linear Models Chromatography Thin Layer medicine.drug |
| Zdroj: | Journal of Chromatography B. :9-14 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2017.09.045 |
| Popis: | An effective thin layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of florfenicol (FF) in pig, chicken and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C18 column using an isocratic procedure with acetonitrile-water (35:65, v/v) at 0.6mL/min. The ultraviolet (UV) detector was set at a wavelength of 225nm. The FF concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y=159075x-15054, r>0.9999) were achieved within the concentration range of 0.05-200μg/mL. The recoveries of FF spiked at levels of 1, 100 and 1000μg/g ranged from 80.6% to 105.3% with the intra-day and inter-day relative standard deviation (RSD) less than 9.3%. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.02 and 0.06mg/kg for pig feedstuffs, 0.02 and 0.07mg/kg for chicken feedstuffs, and 0.02 and 0.05mg/kg for fish feedstuffs, respectively. This reliable, simple and cost-effective method could be applied to the routine monitoring of FF in animal feedstuffs. |
| Databáze: | OpenAIRE |
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