Red blood cells as innovative antigen carrier to induce specific immune tolerance
| Autor: | Nathalie Guerin, Françoise Horand, Alice Banz, Magali Cremel, Yann Godfrin |
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| Rok vydání: | 2012 |
| Předmět: |
Erythrocytes
Ovalbumin medicine.medical_treatment T cell Pharmaceutical Science Antigen-Presenting Cells Succinimides Immune tolerance Mice Immune system Antigen Phagocytosis medicine Immune Tolerance Animals Humans Antigens Antigen-presenting cell Cells Cultured Cellular Senescence Drug Carriers Immunity Cellular biology Macrophages Immunotherapy Immunity Humoral Mice Inbred C57BL Tolerance induction Calcium Ionophores medicine.anatomical_structure Cross-Linking Reagents Immunoglobulin G Immunology biology.protein Antibody |
| Zdroj: | International journal of pharmaceutics. 443(1-2) |
| ISSN: | 1873-3476 |
| Popis: | The route of administration, the dose of antigen as well as the type of antigen-presenting cells (APCs) targeted are important factors to induce immune tolerance. Despite encouraging results obtained in animal models, intravenous injection of soluble antigen is unsuccessful in human clinical trials on autoimmune disease due to inefficient antigen delivery. To improve antigen delivery, we used mouse red blood cells (RBCs) as antigen vehicles to specifically target APCs which are responsible for removal of senescent RBCs after phagocytosis. In this study, we demonstrated that antigen-delivery by RBCs induced a strong decrease in the humoral response compared with the ovalbumin (OVA) free form in mice. In addition, OVA-loaded RBC treated with [bis(sulphosuccinimidyl)] suberate (BS3), a chemical compound known to enhance RBC phagocytosis, induced an inhibition of antigen-specific T cell responses and an increase in the percentage of regulatory T cells. The state of tolerance induced is long lasting, antigen-specific and sufficiently robust to withstand immunization with antigen mixed with cholera toxin adjuvant. This RBC strategy, which does not abolish the immune system, constitutes an attractive approach for induction of tolerance compared to systemic immunosuppressant therapies already in use. |
| Databáze: | OpenAIRE |
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