Dynamic transcriptomes of resistant and susceptible peach lines after infestation by green peach aphids (Myzus persicae Sülzer) reveal defence responses controlled by the Rm3 locus

Autor: Wenfang Zeng, Fan Meili, Niu Liang, Zhiqiang Wang, Lu Zhenhua, Cui Guochao, Qiang Xu, Pan Lei, Guohuai Li
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
lcsh:QH426-470
lcsh:Biotechnology
Population
Locus (genetics)
Aphid resistance
Biology
Chromosomes
Plant
Rm3 locus
03 medical and health sciences
Prunus
Gene Expression Regulation
Plant
lcsh:TP248.13-248.65
Genetics
Animals
Cluster Analysis
MYB
education
Gene
Genetic Association Studies
Disease Resistance
Plant Diseases
Prunus persica
Innate immunity
Aphid
education.field_of_study
Sequence Analysis
RNA
Gene Expression Profiling
Reproducibility of Results
food and beverages
Feeding Behavior
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Peach
Myzus persicae (Sülzer)
WRKY protein domain
lcsh:Genetics
Gene Ontology
Phenotype
030104 developmental biology
Genetic Loci
Aphids
Myzus persicae
Transcriptome analysis
Transcriptome
Plant Shoots
Research Article
Biotechnology
Zdroj: BMC Genomics, Vol 19, Iss 1, Pp 1-15 (2018)
BMC Genomics
ISSN: 1471-2164
Popis: Background The green peach aphid (GPA), Myzus persicae (Sülzer), is a widespread phloem-feeding insect that significantly influences the yield and visual quality of peach [Prunus persica (L.) Batsch]. Single dominant gene (Rm3)-based resistance provides effective management of this invasive pest, although little is known about the molecular responses of plants to GPA feeding. Results To illustrate the molecular mechanisms of monogenic resistance in peach to young tissue-infecting GPAs, aphid-resistant/aphid-susceptible peach lines from a segregating population with Rm3/rm3 and rm3/rm3 genotypes were infested with GPAs for 3 to 72 h. Transcriptome analysis of the infested tissues identified 3854 differentially expressed genes (DEGs). Although the majority of the DEGs in the resistant line also responded to aphid attack in the susceptible line, the overall magnitude of change was greater in the resistant line than in the susceptible line. The enriched gene ontology of the 3854 DEGs involved in plant defence responses included redox situation, calcium-mediated signalling, transcription factor (e.g., WRKY, MYB, and ERF), MAPK signalling cascade, phytohormone signalling, pathogenesis-related protein, and secondary metabolite terms. Of the 53 genes annotated in a 460 kb interval of the rm3 locus, seven genes were differentially expressed between the aphid-resistant and aphid-susceptible peach lines following aphid infestation. Conclusions Together, these results suggest that the Rm3-dependent resistance relies mainly on the inducible expression of defence-related pathways and signalling elements within hours after the initiation of aphid feeding and that the production of specific secondary metabolites from phenylpropanoid/flavonoid pathways can have major effects on peach-aphid interactions. Electronic supplementary material The online version of this article (10.1186/s12864-018-5215-7) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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