Metabolic studies of temperature control strategy on poly(γ-glutamic acid) production in a thermophilic strain Bacillus subtilis GXA-28
| Autor: | Wang Qinglong, Wei Zeng, Guiguang Chen, Zhiqun Liang, Shuangfeng Zheng, Lin Shu |
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| Rok vydání: | 2014 |
| Předmět: |
Environmental Engineering
Glutamic Acid Bioengineering Bacillus subtilis Models Biological chemistry.chemical_compound Species Specificity Biosynthesis Waste Management and Disposal chemistry.chemical_classification biology Renewable Energy Sustainability and the Environment Thermophile Glutamate dehydrogenase Temperature General Medicine Glutamic acid biology.organism_classification Enzyme assay Glucose Enzyme Polyglutamic Acid Biochemistry chemistry Yield (chemistry) Fermentation biology.protein Metabolic Networks and Pathways |
| Zdroj: | Bioresource Technology. 155:104-110 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2013.12.086 |
| Popis: | A thermophilic strain Bacillus subtilis GXA-28 with capability of γ-PGA production was characterized, and its product was identified. The effect of temperatures on cell growth, γ-PGA yield and molecular weight were investigated. Results showed that γ-PGA yield reached 19.92 g/L at 45 °C with a high productivity of 0.91 g/L/h, and the molecular weight reached 3.03 × 10 6 Da. Then, the flux distribution and the key enzyme activities at 2-oxoglutarate branch under specified temperature were determined to illustrate the possible metabolic mechanism contributing to the improved γ-PGA production. Results indicated that the fluxes from iso-citrate to 2-oxoglutarate and from 2-oxoglutarate to glutamate were increased with high activity of isocitrate dehydrogenase and glutamate dehydrogenase, which led to enhance γ-PGA production. This work firstly employed temperature control strategy to improve γ-PGA production, and provided novel information on the metabolic mechanism of γ-PGA biosynthesis in Bacillus species. |
| Databáze: | OpenAIRE |
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