Rat olfactory neurons can utilize the endogenous lectin, L-14, in a novel adhesion mechanism
| Autor: | Douglas N.W. Cooper, Samuel H. Barondes, G. A. Schwarting, N. K. Mahanthappa |
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| Rok vydání: | 1994 |
| Předmět: |
Nervous system
Olfactory system Galectin 1 Olfactory Nerve Molecular Sequence Data Biology Polymerase Chain Reaction Rats Sprague-Dawley Olfactory nerve medicine Cell Adhesion Animals Molecular Biology Cells Cultured DNA Primers Axon Fasciculation Neurons Base Sequence Galactosides Immunohistochemistry Olfactory bulb Cell biology Extracellular Matrix Rats medicine.anatomical_structure Hemagglutinins nervous system Immunology Neuron Olfactory ensheathing glia Laminin Olfactory epithelium Developmental Biology |
| Zdroj: | Development (Cambridge, England). 120(6) |
| ISSN: | 0950-1991 |
| Popis: | L-14 is a divalent, lactosamine-binding lectin expressed in many vertebrate tissues. In the rat nervous system, L-14 expression has been observed previously in restricted neuronal subsets within the dorsal root ganglia and spinal cord. In this study we report that L-14 is expressed by non-neuronal cells in the rat olfactory nerve. We demonstrate that L-14 binds and co-localizes with two ligands in the rat olfactory system: a β-lactosamine-containing glycolipid, and a putative member of the laminin family. The former is expressed on the surfaces of nascent olfactory axons originating from neuron cell bodies in the olfactory epithelium. The latter is present in the extracellular matrix of the axonal path leading to synaptic targets in the olfactory bulb. In vitro, we find that recombinant L-14 promotes primary olfactory neuron adhesion to two laminin family members, and promotes intercellular adhesion. Both activities are dose-dependent, and are independent of integrinmediated mechanisms. We have thus found that L-14 can serve two distinct adhesive functions in vitro, and propose that L-14 in vivo can promote olfactory axon fasciculation by crosslinking adjacent axons and promote axonal adhesion to the extracellular matrix. |
| Databáze: | OpenAIRE |
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