Kemp Elimination Catalyzed by Naturally Occurring Aldoxime Dehydratases
| Autor: | Yasuhisa Asano, Yufeng Miao, Richard Metzner |
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| Rok vydání: | 2016 |
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Molecular Reaction mechanism Stereochemistry 010402 general chemistry 01 natural sciences Biochemistry Catalysis Elimination reaction Catalytic efficiency Molecular Biology Hydro-Lyases chemistry.chemical_classification Acid-Base Equilibrium biology 010405 organic chemistry Organic Chemistry Substrate (chemistry) 0104 chemical sciences Kinetics Enzyme chemistry Biocatalysis Mutation biology.protein Molecular Medicine Enzyme promiscuity |
| Zdroj: | Chembiochem : a European journal of chemical biology. 18(5) |
| ISSN: | 1439-7633 |
| Popis: | Recently, the Kemp elimination reaction has been extensively studied in computational enzyme design of new catalysts, as no natural enzyme has evolved to catalyze this reaction. In contrast to in silico enzyme design, we were interested in searching for Kemp eliminase activity in natural enzymes with catalytic promiscuity. Based on similarities of substrate structures and reaction mechanisms, we assumed that the active sites of naturally abundant aldoxime dehydratases have the potential to catalyze the non-natural Kemp elimination reaction. We found several aldoxime dehydratases that are efficient catalysts of this reaction. Although a few natural enzymes have been identified with promiscuous Kemp eliminase activity, to the best of our knowledge, this is a rare example of Kemp elimination catalyzed by naturally occurring enzymes with high catalytic efficiency. |
| Databáze: | OpenAIRE |
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