The yeast two-hybrid screening technique and its use in the study of protein-protein interactions in apoptosis

Autor:	Andrei Kovalenko, Igor Mett, Mark Boldin, David Wallach, Jacques Camonis, Nikolai Malinin
Rok vydání:	1998
Předmět:	Cloning Programmed cell death Two-hybrid screening Immunology A protein Nucleic Acid Hybridization Apoptosis Biology Yeast Protein–protein interaction Cell biology Fungal Proteins Yeasts Immunology and Allergy Humans Mode of action Signal Transduction
Zdroj:	Current opinion in immunology. 10(2)
ISSN:	0952-7915
Popis:	The yeast two-hybrid technique provides a general approach for cloning cDNAs merely by exploiting the ability of their encoded proteins to bind to a protein of interest. The technique therefore offered a useful access to the analysis of the mechanisms of cell death at the initial stage of their study, when only a few of the proteins involved and very little about their mode of action were known. Conversely, the knowledge of cell death mechanisms gained by this technique provided a useful insight into both the potential and the limitations of this technique.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9066456ef9c09759ac76779c2629155 https://pubmed.ncbi.nlm.nih.gov/9602300 Zobrazit plný text záznamu Full Text from ScienceDirect