Temporal Changes of CB1 Cannabinoid Receptor in the Basal Ganglia as a Possible Structure-Specific Plasticity Process in 6-OHDA Lesioned Rats
| Autor: | Bruna M. Souza, Andréa da Silva Torrão, Caio Henrique Mazucanti, G.P. Chaves-Kirsten, Caroline Cristiane Real, Luiz R.G. Britto |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Calbindins Time Factors Tyrosine 3-Monooxygenase Immunoblotting lcsh:Medicine Substantia nigra Striatum Biology Globus Pallidus Calbindin Basal Ganglia Parkinsonian Disorders Receptor Cannabinoid CB1 Dopamine Internal medicine Basal ganglia mental disorders medicine Animals Rats Wistar lcsh:Science Oxidopamine Multidisciplinary musculoskeletal neural and ocular physiology lcsh:R Dopaminergic Immunohistochemistry Rats FISIOLOGIA Neostriatum Substantia Nigra Globus pallidus Endocrinology nervous system biology.protein lcsh:Q Parvalbumin medicine.drug Research Article |
| Zdroj: | PLoS ONE Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP PLoS ONE, Vol 8, Iss 10, p e76874 (2013) |
| ISSN: | 1932-6203 |
| Popis: | The endocannabinoid system has been implicated in several neurobiological processes, including neurodegeneration, neuroprotection and neuronal plasticity. The CB1 cannabinoid receptors are abundantly expressed in the basal ganglia, the circuitry that is mostly affected in Parkinson's Disease (PD). Some studies show variation of CB1 expression in basal ganglia in different animal models of PD, however the results are quite controversial, due to the differences in the procedures employed to induce the parkinsonism and the periods analyzed after the lesion. The present study evaluated the CB1 expression in four basal ganglia structures, namely striatum, external globus pallidus (EGP), internal globus pallidus (IGP) and substantia nigra pars reticulata (SNpr) of rats 1, 5, 10, 20, and 60 days after unilateral intrastriatal 6-hydroxydopamine injections, that causes retrograde dopaminergic degeneration. We also investigated tyrosine hydroxylase (TH), parvalbumin, calbindin and glutamic acid decarboxylase (GAD) expression to verify the status of dopaminergic and GABAergic systems. We observed a structure-specific modulation of CB1 expression at different periods after lesions. In general, there were no changes in the striatum, decreased CB1 in IGP and SNpr and increased CB1 in EGP, but this increase was not sustained over time. No changes in GAD and parvalbumin expression were observed in basal ganglia, whereas TH levels were decreased and the calbindin increased in striatum in short periods after lesion. We believe that the structure-specific variation of CB1 in basal ganglia in the 6-hydroxydopamine PD model could be related to a compensatory process involving the GABAergic transmission, which is impaired due to the lack of dopamine. Our data, therefore, suggest that the changes of CB1 and calbindin expression may represent a plasticity process in this PD model. |
| Databáze: | OpenAIRE |
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