Variability in Estimating Eccentric Contraction-Induced Muscle Damage and Inflammation in Humans
| Autor: | Mark A. Tarnopolsky, Stuart M. Phillips, Louise J. Beaton |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Contraction (grammar) Physiology Vastus lateralis muscle Cell Count Inflammation Muscle damage Healthy volunteers medicine Humans Eccentric Orthopedics and Sports Medicine Muscle Skeletal Exercise business.industry Macrophages Biopsy Needle Anatomy Torque Female medicine.symptom business Muscle Contraction Muscle contraction |
| Zdroj: | ResearcherID |
| ISSN: | 1066-7814 |
| DOI: | 10.1139/h02-028 |
| Popis: | We studied five young healthy volunteers who performed a "damage protocol" consisting of 240 (24 sets x 10 repetitions/set) maximal isokinetic eccentric muscle contractions (30°/s) on each leg one week apart. Biopsies were taken from the vastus lateralis on two occasions. Two biopsies were taken from within the same muscle 24 h following the damage protocol. On a second occasion a single biopsy was taken from the contralateral leg at 24 h following the same damage protocol. Biopsies at all three sites showed Z-band disruption, much greater (i.e., ∼14-fold) than is typically observed in resting biopsies, with no significant differences (ANOVA) according to site location (within legs or between legs). The within-leg coefficient of variation (CV) was, however, 41 ± 30%, and the between-leg CVs were 57 ± 36% and 68 ± 36%. Macrophage cells were also detected within the muscle, and cell numbers were not statistically different between biopsy sites. However, the within-biopsy CV = 52 ± 19% and the between-biopsy CVs of 34 ± 24% and 48 ± 27%. We conclude that eccentric contraction-induced Z-band streaming and inflammatory cell response, as detected in muscle biopsy samples from humans, is highly variable with a CV of 40-70%. Key words: muscle damage; eccentric exercise; biopsy; coefficient of variation |
| Databáze: | OpenAIRE |
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