Lipid requirements for reconstitution of the delipidated beta-adrenergic receptor and the regulatory protein
| Autor: | Michael Schramm, Sonia Steiner-Mordoch, Zvi Selinger, Jorge Kirilovsky |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Turkeys
Biophysics Synthetic membrane Phospholipid Magnesium Chloride Biology Biochemistry Serine chemistry.chemical_compound Membrane Lipids Structural Biology GTP-Binding Proteins Receptors Adrenergic beta Genetics medicine Choline Animals Magnesium Receptor Molecular Biology Phospholipids Phosphatidylethanolamine Vesicle Erythrocyte Membrane Isoproterenol Cell Biology Thionucleotides Red blood cell Kinetics medicine.anatomical_structure chemistry Guanosine 5'-O-(3-Thiotriphosphate) lipids (amino acids peptides and proteins) Guanosine Triphosphate Soybeans |
| Zdroj: | FEBS letters. 183(1) |
| ISSN: | 0014-5793 |
| Popis: | The role of lipids in the interaction of the β-adrenergic receptor (R) with the regulatory protein (G s ) was investigated. Solubilized preparations of R and of G s from turkey erythrocytes were delipidated by gel filtration. They were subsequently combined and reconstituted by the addition of various lipids. When reconstitution was carried out in the presence of soybean lipids, G s could be fully activated via R by addition of hormone plus GTPγS. In contrast, purified phospholipids or a phospholipid fraction from soybean failed to produce an active system. Fractionation of soybean lipids revealed that acetone-soluble neutral lipids are essential for the reconstitution of a hormone responsive system. The acetone fraction could be replaced by specific neutral lipids such as α-tocopherol or cholesteryl arachidonate while a mixture of phosphatidylethanolamine, -choline and -serine satisfied the phospholipid requirement of the system. Delipidation Reconstitution β-Adrenergic receptor Guanyl nucleotide binding protein Regulatory protein Lipid requirement |
| Databáze: | OpenAIRE |
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