Caste-specific gene expression underlying the differential adult brain development in the honeybee Apis mellifera
| Autor: | V G Coelho Júnior, Ana Durvalina Bomtorin, Zilá Luz Paulino Simões, J J Bruscadin, M T de Oliveira, Lívia Maria Rosatto Moda, D E de Paula Junior, Angel Roberto Barchuk, Daniel Guariz Pinheiro |
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| Přispěvatelé: | UNIFAL-MG, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nervous system media_common.quotation_subject Peptide Hormones Gene Expression brain development diphenism 01 natural sciences 03 medical and health sciences Gene expression honey bee Genetics medicine Morphogenesis Animals Metamorphosis queen Molecular Biology Gene Caspase media_common Life Cycle Stages biology Effector Gene Expression Profiling nervous system Metamorphosis Biological Pupa Brain Gene Expression Regulation Developmental Honey bee Bees Cell biology 010602 entomology 030104 developmental biology medicine.anatomical_structure Insect Science Larva biology.protein Insect Proteins pupa worker Hormone Signal Transduction |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1365-2583 |
| Popis: | Made available in DSpace on 2021-06-25T10:13:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Financiadora de Estudos e Projetos Apis mellifera adult workers feature more developed key brain regions than queens, which allows them to cope with the broad range of duties they need to perform in a colony. However, at the end of larval development, the brain of queens is largely more developed than that of workers. Major morphogenetic changes take place after metamorphosis that shift caste-specific brain development. Here, we tested the hypothesis that this phenomenon is hormonally governed and involves differential gene expression. Our molecular screening approach revealed a set of differentially expressed genes in Pp (first pharate-adult phase) brains between castes mainly coding for tissue remodelling and energy-converting proteins (e.g. hex 70a and ATPsynβ). An in-depth qPCR analysis of the transcriptional behaviour during pupal and pharate-adult developmental stage in both castes and in response to artificially augmented hormone titres of 18 genes/variants revealed that: i. subtle differences in hormone titres between castes might be responsible for the differential expression of the EcR and insulin/insulin-like signalling (IIS) pathway genes; ii. the morphogenetic activity of the IIS in brain development must be mediated by ILP-2, iii. which together with the tum, mnb and caspase system, can constitute the molecular effectors of the caste-specific opposing brain developmental trajectories. Departamento de Biologia Celular e do Desenvolvimento Instituto de Ciências Biomédicas Universidade Federal de Alfenas UNIFAL-MG Faculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual Paulista Departamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo Departamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo Faculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual Paulista FAPESP: 2005/03926-5 FAPESP: 2011/03171-5 CNPq: 406734/2018-6 FAPEMIG: APQ-02134-14 CAPES: Finance Code 001 Financiadora de Estudos e Projetos: MCTI/FINEP/CT-INFRA-PROINFRA 01/2008 Financiadora de Estudos e Projetos: MCTI/FINEP/CT-INFRA-PROINFRA 01/2011 |
| Databáze: | OpenAIRE |
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