A recombinase polymerase amplification assay for rapid detection of Crimean-Congo Haemorrhagic fever Virus infection

Autor: Farida H. Tishkova, Robert J. Watson, Roger Hewson, Babak Afrough, Laura C. Bonney, Manija Mullojonova, Viktoriya Dzhuraeva
Přispěvatelé: Aguilar, Patricia V
Rok vydání: 2017
Předmět:
Tajikistan
0301 basic medicine
Crimean–Congo hemorrhagic fever
Time Factors
Physiology
Recombinase Polymerase Amplification
Artificial Gene Amplification and Extension
Urine
Polymerase Chain Reaction
law.invention
Geographical Locations
Database and Informatics Methods
law
Zoonoses
Pathogen
Neglected tropical diseases
Polymerase chain reaction
lcsh:Public aspects of medicine
Body Fluids
Europe
Infectious Diseases
Molecular Diagnostic Techniques
Hemorrhagic Fever Virus
Crimean-Congo
Crimean-Congo hemorrhagic fever
RNA
Viral
RNA extraction
Anatomy
Sequence Analysis
Nucleic Acid Amplification Techniques
Research Article
Asia
lcsh:Arctic medicine. Tropical medicine
Bioinformatics
lcsh:RC955-962
Point-of-Care Systems
Virulence
Viral diseases
Biology
Research and Analysis Methods
Sensitivity and Specificity
Virus
Recombinases
Middle East
03 medical and health sciences
Extraction techniques
medicine
Humans
Molecular Biology Techniques
Molecular Biology
Medicine and health sciences
Tropical diseases
Hemorrhagic Fevers
Public Health
Environmental and Occupational Health
Biology and Life Sciences
lcsh:RA1-1270
Reverse Transcriptase-Polymerase Chain Reaction
Nucleic acid amplification technique
medicine.disease
Virology
030104 developmental biology
People and Places
Africa
Hemorrhagic Fever
Crimean
Sequence Alignment
Viral hemorrhagic fevers
Zdroj: PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0006013 (2017)
PLoS Neglected Tropical Diseases
ISSN: 1935-2735
1935-2727
DOI: 10.1371/journal.pntd.0006013
Popis: Background Crimean-Congo Haemorrhagic fever Virus (CCHFV) is a rapidly emerging vector-borne pathogen and the cause of a virulent haemorrhagic fever affecting large parts of Europe, Africa, the Middle East and Asia. Methodology/principle findings An isothermal recombinase polymerase amplification (RPA) assay was successfully developed for molecular detection of CCHFV. The assay showed rapid (under 10 minutes) detection of viral extracts/synthetic virus RNA of all 7 S-segment clades of CCHFV, with high target specificity. The assay was shown to tolerate the presence of inhibitors in crude preparations of mock field samples, indicating that this assay may be suitable for use in the field with minimal sample preparation. The CCHFV RPA was successfully used to screen and detect CCHFV positives from a panel of clinical samples from Tajikistan. Conclusions/significance The assay is a rapid, isothermal, simple-to-perform molecular diagnostic, which can be performed on a light, portable real-time detection device. It is ideally placed therefore for use as a field-diagnostic or in-low resource laboratories, for monitoring of CCHF outbreaks at the point-of-need, such as in remote rural regions in affected countries.
Author summary The Crimean-Congo Haemorrhagic fever (CCHF) recombinase polymerase amplification assay is a new, rapid and portable diagnostic method. It has been developed for the detection of infection with CCHF virus (CCHFV), the cause of a deadly haemorrhagic disease in humans and an emerging global health threat. As a rapid diagnostic suitable for use on a portable and lightweight detection device, this has the potential to be used for fast-turnaround diagnosis at the point of need, providing timely results to clinicians at the bedside and preventing the spread of virus in the hospital setting. As a portable diagnostic it would also enable the diagnosis of CCHFV to be taken out of the laboratory during an outbreak, enabling testing in field laboratories or community clinics.
Databáze: OpenAIRE
Externí odkaz: