Induction of de Novo Volatile Terpene Biosynthesis via Cytosolic and Plastidial Pathways by Methyl Jasmonate in Foliage of Vitis vinifera L
| Autor: | Daniela Hampel, and Armin Mosandl, Matthias Wüst |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cytoplasm
Cyclopentanes Mevalonic acid Acetates Vitaceae Gas Chromatography-Mass Spectrometry Terpene chemistry.chemical_compound Mevastatin Biosynthesis medicine Vitis Oxylipins Plastids Methyl jasmonate biology Terpenes General Chemistry biology.organism_classification Fosmidomycin Terpenoid Plant Leaves chemistry Biochemistry Volatilization General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 53:2652-2657 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf040421q |
| Popis: | The terpene biosynthesis in leaves of Vitis vinifera L. cv. Morio Muskat was studied using methyl jasmonate to induce defensive responses in vivo. The experiments demonstrated the strong activation of the de novo biosynthesis of terpenoids via the octadecanoid-signaling cascade and release of the compounds to the gas phase. Feeding experiments with [5,5-2H2]-1-deoxy-d-xylulose and [5,5-2H2]mevalonic acid lactone allowed the investigation of the dynamic allocation of resources via the mevalonic acid and 1-deoxy-d-xylulose/2-C-methyl-d-erythritol 4-phosphate (DOXP/MEP) pathway under induced conditions and after treatment with the specific inhibitors mevastatin and fosmidomycin. The experiments reveal that monoterpenes are almost exclusively synthesized via the DOXP/MEP pathway, whereas sesquiterpenes are generated via both pathways at approximately equal rates. The biosynthesis of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene was not affected by mevastatine or fosmidomycin. Keywords: Vitaceae; 1-deoxy-d... |
| Databáze: | OpenAIRE |
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