In-depth study of homogeneity in DBS using two different techniques: Results from the EBF DBS-microsampling consortium
| Autor: | Nico C van de Merbel, Steve White, Ludovicus Staelens, Matthew Barfield, Ronald de Vries, Mira Doig, Elwin Verheij, Rebecca Broadhurst, Zoe Cobb, Christoph Siethoff, Philip Timmerman, Neil Spooner, Stephen Alaric Williams, Ben van Baar, Bernhard Schmid, Jordi Ortiz |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Bioanalysis
Clinical Biochemistry Analytical chemistry Biomedical Innovation Independent Practice Associations Deoxyglucose Specimen Handling Analytical Chemistry Mice Lacosamide Life Tandem Mass Spectrometry Acetamides Animals Humans Carbon Radioisotopes Hematocrit levels General Pharmacology Toxicology and Pharmaceutics Dried blood Biology Facility Regulation and Control Dried Blood Spot Testing Homogeneity (statistics) Reproducibility of Results General Medicine Rats Europe Substrate type Medical Laboratory Technology Hematocrit QS - Quality & Safety EELS - Earth Environmental and Life Sciences Healthy Living Biomedical engineering |
| Zdroj: | Bioanalysis, 17, 5, 2161-2169 |
| Popis: | Background: At the start of their work, the European Bioanalysis Forum dried blood spots microsampling consortium did not form a dedicated team to investigate the spot homogeneity. However, two teams performed experiments that produced results relating to sample homogeneity. Results: The data, which were produced via two different approaches (a radiolabeled and a nonradiolabeled approach), are highly complementary and demonstrate clear effects on sample inhomogeneity due to the substrate type, compound and hematocrit levels. Conclusion: The results demonstrate that sample inhomogeneity is a significant hurdle to the use of dried blood spots for regulated bioanalysis that should be investigated further in the method establishment phase if the whole spot is not sampled. © 2013 Future Science Ltd. |
| Databáze: | OpenAIRE |
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