Directed Immobilisation of Modificated Galactitol-Dehydrogenase on Gold Electrodes for Electrochemical Cofactor Regeneration

Autor:	Harald Natter, Gerhard Wenz, Jennifer Lenz, Janine Gajdzik, Friedrich Giffhorn, Gert-Wieland Kohring, Rolf Hempelmann
Rok vydání:	2010
Předmět:	Galactitol dehydrogenase biology Chemistry Regeneration (biology) Electrode biology.protein Electrochemistry Combinatorial chemistry Cofactor
Zdroj:	ECS Transactions. 25:13-20
ISSN:	1938-6737 1938-5862
Popis:	A supplemental cysteine-tag modification of the enzyme galactitol dehydrogenase (GatDH), expressed in Escherichia coli has enabled a directed and covalent immobilisation of the protein on gold surfaces without secondary linkers. The successful enzyme immobilisation was verified by SPR measurements, and the activity of the immobilized GatDH was demonstrated by cyclic voltammetry (CV) while NAD+ and the redox mediator 4-carboxy-2,5,7-trinitrofluorenyliden-malonnitrile (CTFM) were both kept in solution. The results represent a proof of concept for the development of electrochemical reactors for the generation of enantiopure fine chemicals as building blocks for pharmaceutical products.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8f975db8b473716634127ea68f12559 https://doi.org/10.1149/1.3309673 Zobrazit plný text záznamu