Wave patterns organize cellular protrusions and control cortical dynamics

Autor: Tatsat Banerjee, Yu Long, Sayak Bhattacharya, Takanari Inoue, Bedri Abubaker-Sharif, Pablo A. Iglesias, Peter N. Devreotes, Yuchuan Miao
Rok vydání: 2019
Předmět:
cell migration
Pattern formation
Biology
Time-Lapse Imaging
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
0302 clinical medicine
complex network
pattern formation
Negative feedback
cellular protrusion
Computer Simulation
Pseudopodia
Cytoskeleton
Quantitative Biology & Dynamical Systems
030304 developmental biology
Positive feedback
0303 health sciences
Microscopy
Confocal
General Immunology and Microbiology
Applied Mathematics
Articles
Models
Theoretical
Actins
Actin Cytoskeleton
Computational Theory and Mathematics
Biophysics
Chemically induced dimerization
Cell Surface Extensions
Lamellipodium
Cell Adhesion
Polarity & Cytoskeleton
General Agricultural and Biological Sciences
Filopodia
030217 neurology & neurosurgery
Information Systems
Signal Transduction
excitable system
Zdroj: Molecular Systems Biology
ISSN: 1744-4292
Popis: Cellular protrusions are typically considered as distinct structures associated with specific regulators. However, we found that these regulators coordinately localize as propagating cortical waves, suggesting a common underlying mechanism. These molecular events fell into two excitable networks, the signal transduction network STEN and the cytoskeletal network CEN with different wave substructures. Computational studies using a coupled‐network model reproduced these features and showed that the morphology and kinetics of the waves depended on strengths of feedback loops. Chemically induced dimerization at multiple nodes produced distinct, coordinated alterations in patterns of other network components. Taken together, these studies indicate: STEN positive feedback is mediated by mutual inhibition between Ras/Rap and PIP2, while negative feedback depends on delayed PKB activation; PKBs link STEN to CEN; CEN includes positive feedback between Rac and F‐actin, and exerts fast positive and slow negative feedbacks to STEN. The alterations produced protrusions resembling filopodia, ruffles, pseudopodia, or lamellipodia, suggesting that these structures arise from a common regulatory mechanism and that the overall state of the STEN‐CEN system determines cellular morphology.
Databáze: OpenAIRE
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