Segregation of microsatellite alleles and residual heterogosity at single loci in homozygous androgenetic common carp (Cyprino carpio L.)
| Autor: | J.J. van der Poel, J. Komen, M. van de Weerd, C.P. Leffering, H. Bovenhuis, M.W.T. Tanck, Arjan P. Palstra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Animal Breeding and Genomics
Common carp Cyprinus Loss of heterozygosity Aquaculture and Fisheries Residual heterozygosity Genetics Fokkerij en Genomica Allele Molecular Biology biology Aquacultuur en Visserij General Medicine Phenotypic trait biology.organism_classification Chiasma Microsatellite allele segregation WIAS Doubled haploids Microsatellite Recombination Linkage analysis Biotechnology |
| Zdroj: | Genome, 44, 743-751 Genome 44 (2001) |
| ISSN: | 0831-2796 |
| Popis: | Thirty-three androgenetic progeny groups of common carp were analysed using 11 microsatellite markers to (i) verify the homozygous status of the 566 androgenetic individuals, (ii) analyse the microsatellite allele segregation, and (iii) study the possible association of microsatellite alleles with phenotypic traits. In total, 92% of the androgenetic individuals proved to be homozygous at all 11 loci. Forty-three of the 47 heterozygous individuals were heterozygous at a single locus only. This heterozygosity was probably due to DNA fragments caused by UV irradiation of the eggs, although the maternal origin of the fragments could not be proved beyond doubt. Screening with 11 microsatellites also revealed two linkage groups, a segregation distortion at two microsatellite loci, and the possible association of some microsatellites with mass, length, stress-related plasma cortisol levels, and basal plasma glucose levels. The success of the linkage and association study could be explained by a low recombination frequency due to high chiasma interference. This would imply a relatively short genetic map for common carp.Key words: doubled haploids, residual heterozygosity, microsatellite allele segregation, linkage analysis, common carp. |
| Databáze: | OpenAIRE |
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