Molecular Approach for the Diagnosis of Blood and Skin Canine Filarioids

Autor: Hacène Medkour, Domenico Otranto, Abdeslam Mekroud, Younes Laidoudi, Idir Bitam, Samia Bedjaoui, Maria Stefania Latrofa, Oleg Mediannikov, Bernard Davoust
Přispěvatelé: Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Higher National Veterinary School, Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Dipartimento di Medicina Veterinaria, Università degli studi di Bari Aldo Moro (UNIBA), Université de Constantine, Superior School of Food Sciences and Food Industries, Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Microorganisms
Microorganisms, MDPI, 2020, 8 (11), ⟨10.3390/microorganisms8111671⟩
Microorganisms, Vol 8, Iss 1671, p 1671 (2020)
Microorganisms, 2020, 8 (11), ⟨10.3390/microorganisms8111671⟩
Volume 8
Issue 11
ISSN: 2076-2607
Popis: The zoonotic Onchocerca lupi and tick-transmitted filarioids of the genus Cercopithifilaria remain less well known due to the difficulties in accessing to skin samples as target tissues. Here, we proposed a molecular approach reliying on multiplex qPCR assays that allow the rapid identification of filarioids from canine blood, skin, and tick samples. This includes two newly developed duplex qPCR tests, the first one targeting filarial and C. grassii DNA (CanFil-C. grassii). and the second qPCR assay designed for the detection of Cercopithifilaria bainae and Cercopithifilaria sp. II DNAs (C. bainae-C.spII). The third one is a triplex TaqMan cox 1 assay targeting DNA of blood microfilariae (e.g., Dirofilaria immitis, Dirofilaria repens and Acanthocheilonema reconditum). The novel duplex qPCRs developed were validated in silico and by screening of known DNA collection. The qPCR assays were also used for screening the blood and tick samples of 72 dogs from Algeria. This allowed the identification of canine filariasis infection with 100% of specificity and 89.47% and 100% of sensitivity from naturally infected blood and tick samples, respectively. The prevalences of 26.39% for D. immitis and 5.56% for both D. repens and A. reconditum were reported in blood and tick samples. Cercopithifilaria DNAs were detected only in tick samples, with a prevalence of 4.17% and 5.56% for C. bainae and Cercopithifilaria sp. II, respectively. Co-infections were diagnosed in 6.94% and 13.89% of blood and tick samples, respectively. Whereas all samples were negative for C. grassii DNA. The use of engorged ticks instead of blood and skin samples could be an easier option for the surveillance of all canine filarioids herein investigated. The multiplex qPCR assays herein validated were shown to be useful in the detection of filarial co-infections by overcoming sequencing of positive samples.
Databáze: OpenAIRE
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