Influence of nanostructural environment and fluid flow on osteoblast-like cell behavior: A model for cell-mechanics studies
| Autor: | Jenneke Klein-Nulend, John A. Jansen, J.J.W.A. van Loon, C.M. Semeins, X.F. Walboomers, Ljupcho Prodanov, J. te Riet |
|---|---|
| Přispěvatelé: | Oral Cell Biology, Maxillofacial Surgery (VUmc), Orale Celbiologie (ORM, ACTA), MKA VUmc (ORM, ACTA), Oral and Maxillofacial Surgery / Oral Pathology, MOVE Research Institute |
| Rok vydání: | 2013 |
| Předmět: |
Bone sialoprotein
Materials science Biomedical Engineering Nanotechnology Microscopy Atomic Force Biochemistry Models Biological 3T3 cells Biomaterials Extracellular matrix Mice Tissue engineering Interstitial fluid Immune Regulation [NCMLS 2] Bone cell medicine Animals Molecular Biology DNA Primers Osteoblasts biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction Substrate (chemistry) General Medicine 3T3 Cells Vinculin Tissue engineering and pathology [NCMLS 3] Nanostructures medicine.anatomical_structure biology.protein Biophysics Biotechnology |
| Zdroj: | Prodanov, L, Semeins, C M, van Loon, J J W A, te Riet, J, Jansen, J A, Klein-Nulend, J & Walboomers, X F 2013, ' Influence of nanostructural environment and fluid flow on osteoblast-like cell behavior: A model for cell-mechanics studies ', Acta Biomaterialia, vol. 9, no. 5, pp. 6653-6662 . https://doi.org/10.1016/j.actbio.2013.02.011 Prodanov, L, Semeins, C M, van Loon, J J W A, te Riet, J, Jansen, J A, Klein-Nulend, J & Walboomers, X F 2013, ' Influence of nanostructural environment and fluid flow on osteoblast-like cell behavior: a model for cell-mechanics studies ', Acta Biomaterialia, vol. 9, no. 5, pp. 6653-6662 . https://doi.org/10.1016/j.actbio.2013.02.011 Acta Biomaterialia, 9(5), 6653-6662. Elsevier BV Acta Biomaterialia, 9, 6653-62 Acta Biomaterialia, 9(5), 6653-6662. Elsevier Acta Biomaterialia, 9, 5, pp. 6653-62 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2013.02.011 |
| Popis: | Item does not contain fulltext Introducing nanoroughness on various biomaterials has been shown to profoundly effect cell-material interactions. Similarly, physical forces act on a diverse array of cells and tissues. Particularly in bone, the tissue experiences compressive or tensile forces resulting in fluid shear stress. The current study aimed to develop an experimental setup for bone cell behavior, combining a nanometrically grooved substrate (200nm wide, 50nm deep) mimicking the collagen fibrils of the extracellular matrix, with mechanical stimulation by pulsatile fluid flow (PFF). MC3T3-E1 osteoblast-like cells were assessed for morphology, expression of genes involved in cell attachment and osteoblastogenesis and nitric oxide (NO) release. The results showed that both nanotexture and PFF did affect cellular morphology. Cells aligned on nanotexture substrate in a direction parallel to the groove orientation. PFF at a magnitude of 0.7Pa was sufficient to induce alignment of cells on a smooth surface in a direction perpendicular to the applied flow. When environmental cues texture and flow were interacting, PFF of 1.4Pa applied parallel to the nanogrooves initiated significant cellular realignment. PFF increased NO synthesis 15-fold in cells attached to both smooth and nanotextured substrates. Increased collagen and alkaline phosphatase mRNA expression was observed on the nanotextured substrate, but not on the smooth substrate. Furthermore, vinculin and bone sialoprotein were up-regulated after 1h of PFF stimulation. In conclusion, the data show that interstitial fluid forces and structural cues mimicking extracellular matrix contribute to the final bone cell morphology and behavior, which might have potential application in tissue engineering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect