Investigation of bacterial interactions using lab on chips
| Autor: | Adele De Ninno, Luca Businaro, Annamaria Gerardino, Francesca Romana Bertani, Daniela Visaggio, Gabriella Cincotti, Lorenzo Nichele, Paolo Visca, Massimiliano Lucidi |
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| Přispěvatelé: | International Conference on Optical Transparent Networks (ICTON), Bari, Italy 2020., Nichele, L., De Ninno, A., Gerardino, A., Bertani, F. R., Visaggio, D., Lucidi, M., Visca, P., Businaro, L., Cincotti, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
biology medicine.drug_class Pseudomonas aeruginosa Microfluidic device Microorganism Antibiotics Bacterial pathogen medicine.disease medicine.disease_cause biology.organism_classification Cystic fibrosis Microbiology Colonisation 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Staphylococcus aureus medicine Antagonism 030217 neurology & neurosurgery Bacteria |
| Zdroj: | ICTON |
| Popis: | Many infections, such as pulmonary infections occurring in cystic fibrosis (CF) patients, are polymicrobial and caused by a combination of different bacterial species. Sometime, a micro-organism predisposes the host to colonisation by other species, and the microbial cooperation or antagonism govern the infectious sequelae. Staphylococcus aureus is one of the first pathogens infecting CF airways, but during adolescence up to 80% of patients become chronically colonised by Pseudomonas aeruginosa, which causes a progressive pulmonary destruction, although antibiotic therapies can improve life expectancy. To investigate the antagonistic interactions between S. aureus and P. aeruginosa, we have designed and fabricated two different lab-on-chips. The first device consists of three parallel compartments, two are filled with different bacterial species while the central one is an agarose barrier, to avoid direct interaction between bacteria. Also the second microfluidic device is composed of three compartments, but the central one is connected to the other two by 260 microchannels; a cell trap is placed at the center of each microchannel to avoid physical contact between bacterial cells in different compartments. We use fluorescent-tagged bacteria and monitor their growth rate inside the microchannels using confocal microscopy. |
| Databáze: | OpenAIRE |
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