Effects of endothelins on renin secretion from isolated mouse renal juxtaglomerular cells
| Autor: | Holger Scholz, Armin Kurtz, Theresia Ritthaler, G. Riegger, Michael Ackermann, Bernhard K. Krämer |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.hormone
Endothelin Receptor Antagonists Male Nitroprusside medicine.medical_specialty Physiology Ratón Viper Venoms In Vitro Techniques Endothelins Calcium Chloride Mice Chloride Channels Internal medicine Renin–angiotensin system Renin medicine Cyclic AMP Animals Vasoconstrictor Agents Secretion Egtazic Acid Kidney Dose-Response Relationship Drug Chemistry Colforsin Juxtaglomerular apparatus Juxtaglomerular Apparatus Peptide Fragments Mice Inbred C57BL Kinetics medicine.anatomical_structure Endocrinology Cell culture Endothelin receptor |
| Zdroj: | The American journal of physiology. 268(1 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | This study was performed to examine the effects of endothelin (ET)-1, ET-2, and ET-3 on renin secretion from cultured mouse renal juxtaglomerular (JG) cells. Although different ETs had no consistent effect on basal renin secretion, they equipotently inhibited adenosine 3',5'-cyclic monophosphate (cAMP)-stimulated renin release with a concentration of approximately 3 nM inhibiting 50% of maximal response. ETs did not significantly affect renin release stimulated by the nitric oxide donor sodium nitroprusside (100 microM) or that stimulated by low [2 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] or high (3 mM CaCl2) extracellular calcium. The inhibitory effect of ETs on cAMP-dependent renin secretion was abolished by lowering extracellular calcium concentration to the nanomolar range. However, the action of ETs was not changed by the ETA receptor antagonist BQ-123 (100 nM) and was mimicked by ETB receptor agonists IRL-1620 (1 microM), sarafotoxin S6b (1 microM), and [Ala1,3,11,15]ET-1 (1 microM). All ETs induced calcium oscillations in JG cells that were dependent on extracellular calcium and were associated with prominent calcium-activated chloride currents. These findings suggest that ETs inhibit rather selectively the cAMP-activated pathway of renin secretion through a calcium-sensitive process. The action of ETs on renal JG cells appears to be mediated via ETB receptors and is presumably related to activation of phospholipase C and subsequent events. |
| Databáze: | OpenAIRE |
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