DNA hybridization for assessment of response of Plasmodium falciparum to chloroquine therapy
| Autor: | Gary H. Campbell, Philippe Deloron, C. Sezibera, A Y Huong, G L McLaughlin |
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| Rok vydání: | 1988 |
| Předmět: |
Microbiology (medical)
Plasmodium falciparum Drug resistance Parasitemia Nucleic acid thermodynamics Chloroquine parasitic diseases medicine Animals Humans biology DNA–DNA hybridization Hybridization probe Infant Nucleic Acid Hybridization DNA medicine.disease biology.organism_classification Molecular biology Malaria Child Preschool Molecular probe DNA Probes medicine.drug Densitometry Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0095-1137 |
| Popis: | We studied the accuracy of PFR1-AP, a synthetic DNA hybridization probe conjugated to alkaline phosphatase, in monitoring Plasmodium falciparum parasitemia during in vivo drug susceptibility surveys. Duplicate blood samples were collected from six children enrolled in a 14-day in vivo chloroquine study in Rwanda. Results obtained by microscopic examination of Giemsa-stained thick blood smears and by DNA hybridization were compared. Both techniques successfully monitored an infection with chloroquine-susceptible parasites and infections exhibiting various levels of resistance to treatment. For each patient, temporal evolution of the microscopic parasite counts and the DNA hybridization signals were closely parallel, although a wide range of rapidly changing levels of parasitemia occurred through the course of the study. This suggests that DNA hybridization assay using PFR1-AP detects P. falciparum parasites sensitively and specifically and is a valuable tool for drug resistance surveys. |
| Databáze: | OpenAIRE |
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