High-throughput microfluidic method to study biofilm formation and host-pathogen interactions in pathogenic Escherichia coli
| Autor: | Mario Jacques, Yannick D. N. Tremblay, Josée Harel, Philippe Vogeleer |
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| Rok vydání: | 2015 |
| Předmět: |
Bacteriological Techniques
Ecology Host (biology) Escherichia coli Proteins Microfluidics Mutant Biofilm Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Bacterial Adhesion Microbiology Pathogenic Escherichia coli In vivo Biofilms Host-Pathogen Interactions medicine Escherichia coli Methods Pathogen Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 81(8) |
| ISSN: | 1098-5336 |
| Popis: | Biofilm formation and host-pathogen interactions are frequently studied using multiwell plates; however, these closed systems lack shear force, which is present at several sites in the host, such as the intestinal and urinary tracts. Recently, microfluidic systems that incorporate shear force and very small volumes have been developed to provide cell biology models that resemble in vivo conditions. Therefore, the objective of this study was to determine if the BioFlux 200 microfluidic system could be used to study host-pathogen interactions and biofilm formation by pathogenic Escherichia coli . Strains of various pathotypes were selected to establish the growth conditions for the formation of biofilms in the BioFlux 200 system on abiotic (glass) or biotic (eukaryotic-cell) surfaces. Biofilm formation on glass was observed for the majority of strains when they were grown in M9 medium at 30°C but not in RPMI medium at 37°C. In contrast, HRT-18 cell monolayers enhanced binding and, in most cases, biofilm formation by pathogenic E. coli in RPMI medium at 37°C. As a proof of principle, the biofilm-forming ability of a diffusely adherent E. coli mutant strain lacking AIDA-I, a known mediator of attachment, was assessed in our models. In contrast to the parental strain, which formed a strong biofilm, the mutant formed a thin biofilm on glass or isolated clusters on HRT-18 monolayers. In conclusion, we describe a microfluidic method for high-throughput screening that could be used to identify novel factors involved in E. coli biofilm formation and host-pathogen interactions under shear force. |
| Databáze: | OpenAIRE |
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