Predominant role of alpha 4-integrins for distinct steps of lymphoma metastasis
| Autor: | David Naor, Alf Hamann, Bernhard Holzmann, Alexandra Lifka, Arne Luz, Petra Jonas, Uwe Gosslar |
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| Rok vydání: | 1996 |
| Předmět: |
Integrins
Integrin alpha4 Spleen Biology Lymphoma T-Cell Metastasis Mice Antigen Antigens CD In vivo Tumor Cells Cultured medicine Animals Neoplasm Invasiveness Neoplasm Metastasis Mice Inbred BALB C Multidisciplinary urogenital system Gene Transfer Techniques medicine.disease BCL10 Lymphoma Disease Models Animal medicine.anatomical_structure Lymphatic system Immunology Cancer research Bone marrow Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:4821-4826 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | To analyze the role of alpha4-integrins in lymphoma metastasis, sublines of the T-cell lymphoma LB were generated by retrovirus-mediated gene transfer that differ exclusively in the expression of alpha4-integrins. Using LB-alpha4 and control LB-NTK cells, we demonstrate that expression of alpha4-integrins strongly suppresses metastasis formation of LB lymphoma cells in secondary lymphoid organs such as spleen, mesenteric and peripheral lymph nodes, or Peyer's patches after i.v. injection into syngeneic BALB/c mice. Moreover, alpha4-integrin expression inhibited development of metastatic tumors in liver, lung, and kidney. Expansion of LB lymphoma cells in bone marrow was not affected by alpha4-integrin expression. In vivo migration assays using 51Cr-labeled lymphoma cells demonstrated that low-metastatic LB-alpha4 cells accumulated with the same efficiency as high-metastatic LB-NTK cells in all target organs examined and were even enriched in mucosal lymphoid organs. Collectively, these results indicate that alpha4-integrins inhibit metastasis formation of lymphoma cells at a stage subsequent to the invasion of target organs. |
| Databáze: | OpenAIRE |
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