Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections
| Autor: | Anu Prathap M. Udayan, Hilary Urena-Saborio, Jess D. Reed, Sundaram Gunasekaran, Sergio Madrigal-Carballo, Emilia Alfaro-Viquez |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Urinary system
Clinical Biochemistry education Virulence 02 engineering and technology Article Microbiology 03 medical and health sciences Pathogenic Escherichia coli nanocomposites Escherichia coli Humans extra-intestinal pathogenic Escherichia coli 030304 developmental biology Detection limit 0303 health sciences Aniline Compounds Bacteria biology Plant Extracts Chemistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification bacteria agglutination Agglutination (biology) Vaccinium macrocarpon Proanthocyanidin Fruit urinary tract infections 0210 nano-technology Biosensor proanthocyanidins TP248.13-248.65 Biotechnology |
| Zdroj: | Biosensors Volume 11 Issue 6 Biosensors, Vol 11, Iss 199, p 199 (2021) |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11060199 |
| Popis: | Consumption of cranberries is associated with the putative effects of preventing urinary tract infections (UTIs). Cranberry proanthocyanidins (PAC) contain unusual double A-type linkages, which are associated with strong interactions with surface virulence factors found on UTI-causing bacteria such as extra-intestinal pathogenic Escherichia coli (ExPEC), depicting in bacterial agglutination processes. In this work, we demonstrated the efficacy of cranberry PAC (200 μg/mL) to agglutinate ExPEC (5.0 × 108 CFU/mL) in vitro as a selective interaction for the design of functionalized biosensors for potential detection of UTIs. We fabricated functionalized screen-printed electrodes (SPEs) by modifying with PAC-polyaniline (PANI) nanocomposites and tested the effectiveness of the PAC-PANI/SPE biosensor for detecting the presence of ExPEC in aqueous suspensions. Results indicated that the PAC-PANI/SPE was highly sensitive (limit of quantification of 1 CFU/mL of ExPEC), and its response was linear over the concentration range of 1–70,000 CFU/mL, suggesting cranberry PAC-functionalized biosensors are an innovative alternative for the detection and diagnosis of ExPEC-associated UTIs. The biosensor was also highly selective, reproducible, and stable. |
| Databáze: | OpenAIRE |
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