Regulatory changes in the formation of chromosomal dihydrofolate reductase causing resistance to trimethoprim
| Autor: | O Sköld, J Flensburg |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Biology
Reductase medicine.disease_cause Microbiology Trimethoprim Plasmid Restriction map Species Specificity Genes Regulator Dihydrofolate reductase Escherichia coli medicine RNA Messenger Molecular Biology chemistry.chemical_classification Base Sequence Drug Resistance Microbial DNA Restriction Enzymes Chromosomes Bacterial Molecular biology Kinetics Tetrahydrofolate Dehydrogenase Enzyme Genes chemistry Genes Bacterial biology.protein Trimethoprim Resistance Research Article Plasmids medicine.drug |
| Zdroj: | Journal of Bacteriology. 159:184-190 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.159.1.184-190.1984 |
| Popis: | High resistance to trimethoprim mediated by the several hundredfold overproduction of the drug target enzyme, dihyrofolate reductase, in a clinically isolated Escherichia coli strain, 1810, was cloned onto several vector plasmids and seemed to be comprised of a single dihydrofolate reductase gene, which by DNA-DNA hybridization and restriction enzyme digestion mapping was very similar to the corresponding gene of E. coli K-12. Determination of mRNA formation in the originally isolated resistant strain and strains with cloned trimethoprim resistance determinant demonstrated an about 15-fold increase in production of dihydrofolate reductase mRNA compared with that in E. coli K-12. This was explained by the occurrence of a promoter up mutation in the resistant isolate accompanied by changes in the restriction enzyme digestion pattern found by comparison with the corresponding pattern from E. coli K-12. |
| Databáze: | OpenAIRE |
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