Simultaneous determination of the carboxylate and lactone forms of 10-hydroxycamptothecin in human serum by restricted-access media high-performance liquid chromatography
| Autor: | Peng-Ling Zhu, Zheng-Ping Jia, Rong Wang, Li-Ting Xu, Jun Ma, Chun-Li Liu |
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| Rok vydání: | 2002 |
| Předmět: |
Clinical Biochemistry
Carboxylic Acids Antineoplastic Agents Biochemistry High-performance liquid chromatography Sensitivity and Specificity Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound Lactones 10-hydroxycamptothecin Humans Carboxylate Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Chemistry Elution Restricted access Reproducibility of Results Cell Biology General Medicine Spectrometry Fluorescence Linear range Calibration Camptothecin Lactone |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 772(2) |
| ISSN: | 1570-0232 |
| Popis: | A simple restricted-access media (RAM) HPLC method for simultaneous determination of the lactone and carboxylate forms of 10-hydroxycamptothecin (HCPT) in human serum was established. Using a RAM Hisep analytical column, serum samples were directly injected into the HPLC system. The eluted peaks of two forms of HCPT were monitored with a fluorescence detector. The separation was completed in 17 min. The linear range was 20–1000 ng/ml, intra-day and inter-day variations being less than 5%. The kinetic equation was introduced according to the analytical results. The equation shows that the course of the HCPT lactone form converting to carboxylate form in human serum at 4 °C is a first-order kinetic course. The concentration of each form at the moment of sampling was calculated by extrapolation. |
| Databáze: | OpenAIRE |
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