Cutaneous Metallothionein Induction by Ultraviolet B Irradiation in Interleukin-6 Null Mice
| Autor: | Hisao Nishimura, Vivienne E. Reeve, Noriko Nishimura, Masahiko Satoh, Chiharu Tohyama |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Ultraviolet Rays
Ratón medicine.medical_treatment TNF Dermatology Biochemistry Mice Proliferating Cell Nuclear Antigen cytokine medicine PCNA Animals Metallothionein Interleukin 6 Molecular Biology Skin biology Interleukin-6 Tumor Necrosis Factor-alpha Cell growth Radioimmunoassay Cell Biology Immunohistochemistry Molecular biology Proliferating cell nuclear antigen Zinc cell proliferation Cytokine Immunology biology.protein Cytokines Tumor necrosis factor alpha Copper Interleukin-1 |
| Zdroj: | Journal of Investigative Dermatology. 114(2):343-348 |
| ISSN: | 0022-202X |
| DOI: | 10.1046/j.1523-1747.2000.00862.x |
| Popis: | The mediators of cutaneous metallothionein induction by ultraviolet radiation have not been defined. In this study we sought to identify cytokines that might be involved. We examined the role of interleukin-6, using the IL-6 null (IL-6-/-) mouse, which has been observed to be highly sensitive to ultraviolet radiation damage. Whereas cutaneous metallothionein concentration, measured by radioimmunoassay, began to rise in wild-type (IL-6+/+) mice by 12 h after ultraviolet irradiation, there was a significant delay in the IL-6-/- mice until 48 h after UV irradiation. Immunohistologically, metallothionein appeared in IL-6+/+ mice at 24 h in dermal fibroblasts, and then by 48 h in epidermal basal keratinocytes, with intensity increasing until 72 h, and was coincident with proliferating cell nuclear antigen-positive staining. Corresponding metallothionein expression in IL-6-/- mouse skin was significantly delayed. Serum interleukin-6 was elevated in IL-6+/+ mice following ultraviolet irradiation, with peak concentration at 4 h, but no increase in serum interleukin-1beta was found in either IL-6+/+ or IL-6-/- mice. Interestingly, tumor necrosis factor alpha concentration in serum was elevated at 12 h postirradiation in IL-6+/+ mice, but there was an earlier (at 4 and 8 h) time-dependent increase in tumor necrosis factor alpha in serum of the IL-6-/- mice. Skin zinc and copper concentrations were not altered by ultraviolet irradiation in either IL-6+/+ or IL-6-/- mice. The results suggest that interleukin-6 may be a very early mediator of cutaneous metallothionein induction by ultraviolet radiation, but that this role is possibly assumed by alternative cytokines like tumor necrosis factor alpha when interleukin-6 is deficient. |
| Databáze: | OpenAIRE |
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