Identification and characterization of a new Leishmania major specific 3′nucleotidase/nuclease protein

Autor: Yvan Boublik, Mehdi Chenik, Hechmi Louzir, Yosser Ben Achour-Chenik, Ahlem Aamouri, Inès Lakhal-Naouar, Abdellatif Fattoum, Mounira Meddeb
Přispěvatelé: Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Plateforme de Production des Protéines Recombinantes, Université de Montpellier (UM), Département d'Histologie et de Cytogénétique - Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)
Rok vydání: 2008
Předmět:
[SDV]Life Sciences [q-bio]
Protozoan Proteins
MESH: Amino Acid Sequence
MESH: Nucleotidases
Biochemistry
Leishmania mexicana
MESH: Leishmania major
0302 clinical medicine
Nucleotidases
purine scavenging pathway
MESH: Animals
Leishmania major
MESH: Phylogeny
MESH: Protozoan Proteins
Peptide sequence
Phylogeny
Leishmania
0303 health sciences
3 ` nucleotidase/nuclease
biology
medicine.diagnostic_test
MESH: Purines
MESH: Gene Expression Regulation
Molecular Sequence Data
030231 tropical medicine
sandfly
Biophysics
survival
03 medical and health sciences
Western blot
Nucleotidase
medicine
Animals
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Amino Acid Sequence
Molecular Biology
Gene
030304 developmental biology
Nuclease
MESH: Molecular Sequence Data
Cell Membrane
Cell Biology
biology.organism_classification
Molecular biology
Gene Expression Regulation
Purines
biology.protein
MESH: Cell Membrane
Zdroj: Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications, Elsevier, 2008, 375 (1), pp.54-8. ⟨10.1016/j.bbrc.2008.07.099⟩
ISSN: 0006-291X
1090-2104
DOI: 10.1016/j.bbrc.2008.07.099
Popis: International audience; We report the characterization of a new Leishmania major gene, lmaj3'nt/nu, encoding a 382 amino acids protein, Lmaj3'NT/NU, that belongs to the 3'nucleotidase/nuclease family. Interestingly, sequence and phylogenetic analysis show that this protein is Leishmania major specific and thus constitutes a new 3'nucleotidase/nuclease subgroup. Lmaj3'NT/NU displays nuclease enzymatic activity and Western blot analysis shows that it is exclusively expressed in promastigotes. Immunofluorescence microscopy using a specific anti-Lmaj3'NT/NU shows that the protein has a plasma membrane localization. Surprisingly, contrary to the previously described Leishmania mexicana 3'NT/NU, lmaj3'nt/nu is not up-regulated when parasites are cultured under purine starvation conditions. Together, these findings suggest Lmaj3'NT/NU may constitute a new important compound of the L. major purine scavenging pathway and could be involved in sandfly parasite survival and colonization.
Databáze: OpenAIRE
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