A Chinese herbal decoction, Jian-Pi-Yi-Shen, regulates the expressions of erythropoietin and pro-inflammatory cytokines in cultured cells
| Autor: | Airong Qi, Jianping Chen, Tina T. X. Dong, Amy G. W. Gong, Shunmin Li, Tiegang Yi, Xiaoyan Liu, Karl Wah Keung Tsim, Zhonggui Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pro-inflammatory cytokines Pharmacology Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Immune system medicine Humans Erythropoietin Chemistry HEK 293 cells Interleukin lcsh:Other systems of medicine General Medicine Transfection lcsh:RZ201-999 Haematopoiesis HEK293 Cells 030104 developmental biology Gene Expression Regulation Complementary and alternative medicine 030220 oncology & carcinogenesis Cytokines Tumor necrosis factor alpha Herbal medicine Research Article Drugs Chinese Herbal medicine.drug |
| Zdroj: | BMC Complementary and Alternative Medicine, Vol 18, Iss 1, Pp 1-8 (2018) BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-018-2146-4 |
| Popis: | Background A Chinese herbal formula, namely Jian-Pi-Yi-Shen (JPYS), has been clinically prescribed for patients with chronic kidney disease associated-anemia, and which can improve the patient’s immunological system. However, the mechanisms of JPYS involved in anemia and immune response have not been investigated. To study the role of JPYS in regulating hematopoietic and immunological functions, we investigated its activities on the expressions of erythropoietin and pro-inflammatory cytokines in cultured cells. Methods The standardized herbal extracts of JPYS (0–30 μg/ml) were applied onto cultured cells for 24–48 h. Total RNA was collected from the treated cells and subjected to real-time quantitative PCR analysis. Cultured HEK293T cells, transfected with a construct composed of hypoxia response element tagged with a luciferase gene, i.e. pHRE-Luc, were treated with JPYS extracts (1–30 μg/ml) for 24 h. The cell lysates were subjected to luciferase assay. Results The treatment with JPYS extract onto cultured HEK293T cells induced erythropoietin expression in a dose-dependent manner, having the highest response by ~ 50% of increase. In parallel, application of JPYS extract for 24 h stimulated expressions of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in cultured RAW 264.7 macrophages. In contrast, the pretreatment with JPYS extract suppressed expressions of IL-1β, IL-6, and TNF-α in lipopolysaccharide-induced macrophages. Conclusions These results confirmed the hematopoietic function of JPYS in regulating erythropoietin expression, as well as the bidirectional immune-modulatory roles of JPYS by regulating the expression of pro-inflammatory cytokines in cultures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink