Applications of imaging flow cytometry in the diagnostic assessment of red cell membrane disorders
| Autor: | Tejashree Anil More, Bhavik Dalal, Prabhakar Kedar, Rati Devendra, Aruna Shankarkumar, Prashant Warang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Histology Erythrocytes Adolescent Hereditary elliptocytosis Spherocytosis Hereditary Pathology and Forensic Medicine Flow cytometry Hereditary spherocytosis 03 medical and health sciences Young Adult 0302 clinical medicine Microscopy medicine Humans education Child Aged education.field_of_study medicine.diagnostic_test Red Cell Elliptocytes Chemistry Cell Membrane Elliptocytosis Hereditary Infant Cell Biology Middle Aged medicine.disease Flow Cytometry Red blood cell 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Child Preschool Female Quantitative analysis (chemistry) Biomedical engineering |
| Zdroj: | Cytometry. Part B, Clinical cytometryREFERENCES. 98(3) |
| ISSN: | 1552-4957 |
| Popis: | BACKGROUND Red cell membranopathies refers to phenotypically and morphologically heterogeneous disorders. High throughput imaging flow cytometry (IFC) combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy to produce high content image analysis with quantitative analysis. We have evaluated the applications of IFC to examine both the morphology as well as fluorescence signal intensity in red cell membranopathies. METHODS Fluorescence intensity of eosin-5-maleimide (EMA) labeled red cells was measured for diagnosis of RBC membrane protein defect on Amnis ImageStreamX followed by Image analysis on IDEAS software to study features such as circularity and shape ratio. RESULTS The hereditary spherocytosis (HS) group showed significantly decreased MFI (52,800 ± 9,100) than normal controls (81,100 ± 4,700) (p |
| Databáze: | OpenAIRE |
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