Pharmacologically active microcarriers for endothelial progenitor cell support and survival

Autor: Astrid Parenti, Claudia Musilli, Claudia N. Montero-Menei, Jean-Pierre Karam, Claudio Muscari, Sara Paccosi, Alessandro Mugelli
Přispěvatelé: Ingénierie de la vectorisation particulaire, Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Geriatric Research, Education and Clinical Center and Research Services, Bruce W. Carter Veterans Affairs Medical Center, Micro et Nanomédecines Biomimétiques (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), Musilli C, Karam JP, Paccosi S, Muscari C, Mugelli A, Montero-Menei CN, Parenti A
Rok vydání: 2012
Předmět:
Vascular Endothelial Growth Factor A
Cell Survival
[SDV]Life Sciences [q-bio]
Blotting
Western
Poly (ADP-Ribose) Polymerase-1
Neovascularization
Physiologic
Pharmaceutical Science
030204 cardiovascular system & hematology
Biology
Endothelial progenitor cell
Early endothelial progenitor cell
VEGF-A
03 medical and health sciences
0302 clinical medicine
Polylactic Acid-Polyglycolic Acid Copolymer
Cell Movement
Cell Adhesion
Human Umbilical Vein Endothelial Cells
Pharmacologically active microcarrier
Humans
Lactic Acid
Viability assay
Phosphorylation
Progenitor cell
Cell adhesion
Cell Proliferation
030304 developmental biology
Mitogen-Activated Protein Kinase 1
0303 health sciences
Mitogen-Activated Protein Kinase 3
Tissue Scaffolds
Cell growth
Stem Cells
Endothelial Cells
Cell migration
General Medicine
Extracellular Matrix
Cell biology
Endothelial stem cell
Gene Expression Regulation
Endothelial cell proliferation and migration
embryonic structures
Immunology
Poly(ADP-ribose) Polymerases
Stem cell
Polyglycolic Acid
Biotechnology
Zdroj: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, 2012, 81 (3), pp.609-16. ⟨10.1016/j.ejpb.2012.04.014⟩
ISSN: 0939-6411
1873-3441
DOI: 10.1016/j.ejpb.2012.04.014
Popis: International audience; The regenerative potential of endothelial progenitor cell (EPC)-based therapies is limited due to poor cell viability and minimal retention following application. Neovascularization can be improved by means of scaffolds supporting EPCs. The aim of the present study was to investigate whether human early EPCs (eEPCs) could be efficiently cultured on pharmacologically active microcarriers (PAMs), made with poly(d,l-lactic-coglycolic acid) and coated with adhesion/extracellular matrix molecules. They may serve as a support for stem cells and may be used as cell carriers providing a controlled delivery of active protein such as the angiogenic factor, vascular endothelial growth factor-A (VEGF-A). eEPC adhesion to fibronectin-coated PAMs (FN-PAMs) was assessed by means of microscopic evaluation and by means of Alamar blue assay. Phospho ERK(1/2) and PARP-1 expression was measured by means of Western blot to assess the survival effects of FN-PAMs releasing VEGF-A (FN-VEGF-PAMs). The Alamar blue assay or a modified Boyden chamber assay was employed to assess proliferative or migratory capacity, respectively. Our data indicate that eEPCs were able to adhere to empty FN-PAMs within a few hours. FN-VEGF-PAMs increased the ability of eEPCs to adhere to them and strongly supported endothelial-like phenotype and cell survival. Moreover, the release of VEGF-A by FN-PAMs stimulated in vitro HUVEC migration and proliferation. These data strongly support the use of PAMs for supporting eEPC growth and survival and for stimulating resident mature human endothelial cells.
Databáze: OpenAIRE
Externí odkaz: