Differential screening of phage-ab libraries by oligonucleotide microarray technology
| Autor: | Alessandra Vitelli, Riccardo Cortese, Maria Raffaella Ambrosio, Emanuele de Rinaldis, Laura Orsatti, Fabio Palombo, Alfredo Nicosia, Francesca Magistri, Virginia Ammendola, Maurizio Nuzzo, Alessandra De Pra, Mirko Arcuri, Stefano Colloca, Helenia Ansuini, Claudia Santini, Monica Pezzanera, Valentina Viti, Philippe Neuner, Saverio Giampaoli, Gaetano Barbato, Andrea Sbardellati, Maria Giulia Bigotti, Fabio Talamo, Paolo Uva, Anna Demartis, Annamaria Assunta Nasti, Armin Lahm, Paolo Monaci, Agostino Cirillo, Alessandro Bellini, Alessandra Luzzago, Giuseppe Silvestre |
|---|---|
| Přispěvatelé: | P., Monaci, A., Luzzago, C., Santini, A. D., Pra, M., Arcuri, F., Magistri, A., Bellini, H., Ansuini, M., Ambrosio, V., Ammendola, M. G., Bigotti, A., Cirillo, M., Nuzzo, A. A., Nasti, P., Neuner, L., Orsatti, M., Pezzanera, A., Sbardellati, G., Silvestre, P., Uva, V., Viti, G., Barbato, S., Colloca, A., Demarti, E. D., Rinaldi, S., Giampaoli, A., Lahm, F., Palombo, F., Talamo, A., Vitelli, Nicosia, Alfredo, R., Cortese |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Phagemid
Immunology Population lcsh:Medicine Oncology/Gastrointestinal Cancers Enzyme-Linked Immunosorbent Assay Mice Animals Bacteriophages education Receptor lcsh:Science Molecular Biology Oligonucleotide Array Sequence Analysis Cloning Mice Inbred BALB C education.field_of_study Multidisciplinary biology DNA–DNA hybridization lcsh:R Nucleic acid sequence Computational Biology Antibodies Monoclonal Membrane Proteins Surface Plasmon Resonance Molecular biology Immunoglobulin G biology.protein lcsh:Q Antibody DNA microarray Research Article Biotechnology |
| Zdroj: | PLoS ONE, Vol 3, Iss 1, p e1508 (2008) PLoS ONE Monaci, P, Luzzago, A, Santini, C, De Pra, A, Arcuri, M, Magistri, F, Bellini, A, Ansuini, H, Ambrosio, M, Ammendola, V, Bigotti, M G, Cirillo, A, Nuzzo, M, Nasti, A A, Neuner, P, Orsatti, L, Pezzanera, M, Sbardellati, A, Silvestre, G, Uva, P, Viti, V, Barbato, G, Colloca, S, Demartis, A, De Rinaldis, E, Giampaoli, S, Lahm, A, Palombo, F, Talamo, F, Vitelli, A, Nicosia, A & Cortese, R 2008, ' Differential screening of phage-ab libraries by oligonucleotide microarray technology ', PLoS ONE, vol. 3, no. 1, e1508 . https://doi.org/10.1371/journal.pone.0001508 |
| ISSN: | 1932-6203 |
| Popis: | A novel and efficient tagArray technology was developed that allows rapid identification of antibodies which bind to receptors with a specific expression profile, in the absence of biological information. This method is based on the cloning of a specific, short nucleotide sequence (tag) in the phagemid coding for each phage-displayed antibody fragment (phage-Ab) present in a library. In order to set up and validate the method we identified about 10,000 different phage-Abs binding to receptors expressed in their native form on the cell surface (10 k Membranome collection) and tagged each individual phage-Ab. The frequency of each phage-Ab in a given population can at this point be inferred by measuring the frequency of its associated tag sequence through standard DNA hybridization methods. Using tiny amounts of biological samples we identified phage-Abs binding to receptors preferentially expressed on primary tumor cells rather than on cells obtained from matched normal tissues. These antibodies inhibited cell proliferation in vitro and tumor development in vivo, thus representing therapeutic lead candidates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|