Mild heat induces a distinct 'eustress' response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis
| Autor: | Begüm Peksel, Imre Gombos, Mária Péter, László Vigh, Ádám Tiszlavicz, Mario Brameshuber, Gábor Balogh, Gerhard J. Schütz, Ibolya Horváth, Zsolt Török |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Hot Temperature Fever Cell Survival lcsh:Medicine CHO Cells Article 03 medical and health sciences Cricetulus Cricetinae Heat shock protein Lipidomics Fluorescence microscope medicine Animals Heat shock lcsh:Science Heat-Shock Proteins Multidisciplinary Chemistry Chinese hamster ovary cell lcsh:R Adaptation Physiological Cell biology 030104 developmental biology Proteostasis Shock (circulatory) lcsh:Q medicine.symptom Heat-Shock Response Homeostasis |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The current research on cellular heat stress management focuses on the roles of heat shock proteins (HSPs) and the proteostasis network under severe stress conditions. The mild, fever-type stress and the maintenance of membrane homeostasis are less well understood. Herein, we characterized the acute effect of mild, fever-range heat shock on membrane organization, and HSP synthesis and localization in two mammalian cell lines, to delineate the role of membranes in the sensing and adaptation to heat. A multidisciplinary approach combining ultrasensitive fluorescence microscopy and lipidomics revealed the molecular details of novel cellular “eustress”, when cells adapt to mild heat by maintaining membrane homeostasis, activating lipid remodeling, and redistributing chaperone proteins. Notably, this leads to acquired thermotolerance in the complete absence of the induction of HSPs. At higher temperatures, additional defense mechanisms are activated, including elevated expression of molecular chaperones, contributing to an extended stress memory and acquired thermotolerance. |
| Databáze: | OpenAIRE |
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