Mitochondrial and Metabolic Gene Expression in the Aged Rat Heart
| Autor: | Joseph J. Sepe, Gary M. Diffee, Judd M. Aiken, Susan H. McKiernan, Gregory P. Barton |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty mitochondrial biogenesis Physiology glucose metabolism Peroxisome proliferator-activated receptor 030204 cardiovascular system & hematology Biology Carbohydrate metabolism lcsh:Physiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Physiology (medical) medicine Beta oxidation Original Research chemistry.chemical_classification Fatty acid metabolism lcsh:QP1-981 AMPK Fatty acid 030104 developmental biology Endocrinology chemistry Mitochondrial biogenesis fatty acid metabolism gene expression Peroxisome proliferator-activated receptor alpha exercise training |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 7 (2016) |
| ISSN: | 1664-042X |
| Popis: | Aging is associated with a decline in cardiac function. Exercise intervention has been suggested as a way to improve this decrement. Age-related decline in cardiac function is associated with decreases in fatty acid oxidation, mitochondrial function and AMP-activated protein kinase (AMPK) activity. The molecular mechanisms involved with age-related changes in mitochondrial function and substrate metabolism are poorly understood. We determined gene expression differences in hearts of Young (6 mo), Old (33 mo), and old exercise trained (Old + EXE) (34 mo) FBN rats, using Qiagen PCR arrays for Glucose, Fatty acid, and Mitochondrial metabolism. Old rats demonstrated decreased (p < 0.05) expression for key genes in fatty acid oxidation, mitochondrial function, and AMPK signaling. There were no differences in the expression of genes involved in glucose metabolism with age. These gene expression changes occurred prior to altered protein translation as we found no differences in the protein content of peroxisome proliferator activated receptor gamma, coactivators 1 alpha (PGC-1α), peroxisome proliferator activated receptor alpha (PPARα), and AMPKα2 between young and old hearts. Four months of exercise training did not attenuate the decline in the gene expression in aged hearts. Despite this lack of change in gene expression, exercise-trained rats demonstrated increased exercise capacity compared to their sedentary counterparts. Taken together, our results show that differential expression of genes associated with fatty acid metabolism, AMPK signaling and mitochondrial function are superfluous and decrease in the aging heart which may play a role in age-related declines in fatty acid oxidation, AMPK activity and mitochondrial function in the heart. |
| Databáze: | OpenAIRE |
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