Expression and localization of dystrophin in cultured neonatal rat cardiac myocytes
| Autor: | Hirotaka Miyazato, Masato Maeda, Sadatoshi Biro, Manabu Setoguchi, Hiromichi Tanaka, Shoichiro Nakao |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cellular differentiation
Heart Ventricles Blotting Western Biophysics Biochemistry Dystrophin Western blot medicine Myocyte Animals Rats Wistar Molecular Biology Cells Cultured biology medicine.diagnostic_test Myocardium Cell Biology Molecular biology Immunohistochemistry Staining Rats Animals Newborn Cell culture biology.protein Antibody |
| Zdroj: | Biochemical and biophysical research communications. 194(3) |
| ISSN: | 0006-291X |
| Popis: | We examined the expression and localization of dystrophin in cultured neonatal rat cardiac myocytes by Western blot analysis and immunohistochemistry using an antidystrophin antibody. Western blot analysis showed a single band with a molecular weight of about 400 kd. Immunohistochemical staining also detected dystrophin in cultured cardiac myocytes, and it was found primarily around the nucleus on day 2 of culture, the area and degree of staining increased on days 4 and 7, and most cells were stained almost entirely on day 14. We conclude that dystrophin also is expressed in cultured neonatal rat cardiac myocytes, and that it increases with time, and extends from the perinuclear area to the whole cell. |
| Databáze: | OpenAIRE |
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