Single-molecule sequencing of long DNA molecules allows high contiguityde novogenome assembly for the fungus fly,Sciara coprophila
| Autor: | Susan A. Gerbi, Nanyan Lu, John M. Urban, Jacob E. Bliss, C. M. Coleman, Michael S. Foulk, Reza Mazloom, Susan J. Brown, Allan C. Spradling |
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| Rok vydání: | 2020 |
| Předmět: |
Whole genome sequencing
0303 health sciences Sciara Sequence assembly Genomics Genome project Computational biology Biology biology.organism_classification Genome 03 medical and health sciences 0302 clinical medicine Minion Nanopore sequencing 030217 neurology & neurosurgery 030304 developmental biology |
| Popis: | The lower Dipteran fungus fly,Sciara coprophila, has many unique biological features. For example,Sciaraundergoes paternal chromosome elimination and maternal X chromosome nondisjunction during spermatogenesis, paternal X elimination during embryogenesis, intrachromosomal DNA amplification of DNA puff loci during larval development, and germline-limited chromosome elimination from all somatic cells. Paternal chromosome elimination inSciarawas the first observation of imprinting, though the mechanism remains a mystery. Here, we present the first draft genome sequence forSciara coprophilato take a large step forward in aiding these studies. We approached assembling theSciaragenome using multiple sequencing technologies: PacBio, Oxford Nanopore MinION, and Illumina. To find an optimal assembly using these datasets, we generated 44 Illumina assemblies using 7 short-read assemblers and 50 long-read assemblies of PacBio and MinION sequence data using 6 long-read assemblers. We ranked assemblies using a battery of reference-free metrics, and scaffolded a subset of the highest-ranking assemblies using BioNano Genomics optical maps. RNA-seq datasets from multiple life stages and both sexes facilitated genome annotation. Moreover, we anchored nearly half of theSciaragenome sequence into chromosomes. Finally, we used the signal level of both the PacBio and Oxford Nanopore data to explore the presence or absence of DNA modifications in theSciaragenome since DNA modifications may play a role in imprinting inSciara, as they do in mammals. These data serve as the foundation for future research by the growing community studying the unique features of this emerging model system. |
| Databáze: | OpenAIRE |
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