Effect of D-alpha-tocopherol on tubular nephron acidification by rats with induced diabetes mellitus
| Autor: | F. T. Barbosa, F. Zaladek Gil, Maria de Fátima Cavanal, G. Nascimento Gomes, Margarida de Mello Aires, R. F. Radaeli |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Physiology Kidney Glomerulus alpha-Tocopherol Nephron Biochemistry Antioxidants chemistry.chemical_compound Diabetic Nephropathies General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Acidosis lcsh:R5-920 General Neuroscience Diabetes Acidosis Renal Tubular General Medicine Kidney Tubules medicine.anatomical_structure medicine.symptom lcsh:Medicine (General) Glomerular Filtration Rate medicine.drug Vitamin medicine.medical_specialty Bicarbonate Immunology Biophysics Renal function Diabetes Mellitus Experimental Nephropathy Diabetes mellitus Internal medicine medicine Animals Rats Wistar business.industry Nephrons Cell Biology medicine.disease Streptozotocin Rats Endocrinology lcsh:Biology (General) chemistry D-alpha-tocopherol business |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 38, Issue: 7, Pages: 1043-1051, Published: JUL 2005 Brazilian Journal of Medical and Biological Research, Vol 38, Iss 7, Pp 1043-1051 (2005) Brazilian Journal of Medical and Biological Research v.38 n.7 2005 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| ISSN: | 0100-879X |
| Popis: | The objective of the present study was to determine if treatment of diabetic rats with D-alpha-tocopherol could prevent the changes in glomerular and tubular function commonly observed in this disease. Sixty male Wistar rats divided into four groups were studied: control (C), control treated with D-alpha-tocopherol (C + T), diabetic (D), and diabetic treated with D-alpha-tocopherol (D + T). Treatment with D-alpha-tocopherol (40 mg/kg every other day, ip) was started three days after diabetes induction with streptozotocin (60 mg/kg, ip). Renal function studies and microperfusion measurements were performed 30 days after diabetes induction and the kidneys were removed for morphometric analyses. Data are reported as means +/- SEM. Glomerular filtration rate increased in D rats but decreased in D + T rats (C: 6.43 +/- 0.21; D: 7.74 +/- 0.45; D + T: 3.86 +/- 0.18 ml min-1 kg-1). Alterations of tubular acidification observed in bicarbonate absorption flux (JHCO3) and in acidification half-time (t/2) in group D were reversed in group D + T (JHCO3, C: 2.30 +/- 0.10; D: 3.28 +/- 0.22; D + T: 1.87 +/- 0.08 nmol cm-2 s-1; t/2, C: 4.75 +/- 0.20; D: 3.52 +/- 0.15; D + T: 5.92 +/- 0.19 s). Glomerular area was significantly increased in D, while D + T rats exhibited values similar to C, suggesting that the vitamin prevented the hypertrophic effect of hyperglycemia (C: 8334.21 +/- 112.05; D: 10,217.55 +/- 100.66; D + T: 8478.21 +/- 119.81 microm(2)). These results suggest that D-alpha-tocopherol is able to protect rats, at least in part, from the harmful effects of diabetes on renal function. |
| Databáze: | OpenAIRE |
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