Only One Splice Variant of the Human TAZ Gene Encodes a Functional Protein with a Role in Cardiolipin Metabolism
| Autor: | Riekelt H. Houtkooper, Ronald Ja Wanders, Peter G. Barth, Fredoen Valianpour, Frédéric M. Vaz |
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| Přispěvatelé: | Laboratory Genetic Metabolic Diseases, Paediatric Neurology, Paediatric Metabolic Diseases |
| Rok vydání: | 2003 |
| Předmět: |
Genetic Linkage
Tafazzin Biochemistry Mass Spectrometry chemistry.chemical_compound Exon Cardiolipin Chromatography High Pressure Liquid Growth Disorders Genetics biology Barth syndrome Exons Syndrome lipids (amino acids peptides and proteins) Cardiomyopathies DNA Complementary Neutropenia Cardiolipins Molecular Sequence Data Saccharomyces cerevisiae Genes Recessive Antibodies Muscular Diseases medicine Humans splice Amino Acid Sequence Molecular Biology Gene Chromosomes Human X Models Genetic Sequence Homology Amino Acid Genetic Complementation Test Wild type Proteins DNA Cell Biology medicine.disease biology.organism_classification Carbon Alternative Splicing Models Chemical chemistry Mutagenesis Mutation biology.protein Acyltransferases Transcription Factors |
| Zdroj: | Journal of biological chemistry, 278(44), 43089-43094. American Society for Biochemistry and Molecular Biology Inc. |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m305956200 |
| Popis: | Barth syndrome (BTHS) is an X-linked recessive disorder caused by mutations in the TAZ gene and is characterized by cardiomyopathy, short stature, neutropenia, and 3-methylglutaconic aciduria. Recently it was found that BTHS patients exhibit a profound cardiolipin deficiency although the biosynthetic capacity to synthesize this lipid from its precursor phosphatidylglycerol is entirely normal. Like BTHS patients, a Saccharomyces cerevisiae strain, in which the yeast orthologue of the human TAZ gene has been disrupted, exhibits an abnormal cardiolipin profile as determined by tandem mass spectrometry. Additionally, this yeast strain grows poorly on non-fermentable carbon sources. We have used both properties of this yeast disruptant as a read-out system to test the physiological functionality of each of 12 different splice variants that have been reported for the human TAZ gene. Our results demonstrate that only the splice variant lacking exon 5 was able to complement the retarded growth of the yeast disruptant on selective plates and restore the cardiolipin profile to the wild type pattern. We conclude that this splice variant most likely represents the only physiologically important mRNA, at least with regard to cardiolipin metabolism. |
| Databáze: | OpenAIRE |
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