Yokukansan (Kampo medicinal formula) prevents the development of morphine tolerance by inhibiting the secretion of orexin A
| Autor: | Mana Tsukada, Yasuaki Kanada, Hiroki Suga, Haruka Takemura, Hitoshi Mera, Masataka Sunagawa, Ayami Katayama, Yuko Akanuma, Tadashi Hisamitsu, Takahiro Ono |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Kampo Analgesic Yokukansan Pharmacology 03 medical and health sciences Subcutaneous injection Orexin-A 0302 clinical medicine Medicine Hot plate test lcsh:Miscellaneous systems and treatments Orexin A business.industry lcsh:RZ409.7-999 Morphine tolerance 030104 developmental biology Nociception Complementary and alternative medicine nervous system Kampo medicine Morphine Original Article business Astrocyte 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Integrative Medicine Research, Vol 7, Iss 2, Pp 141-148 (2018) Integrative Medicine Research |
| ISSN: | 2213-4220 |
| Popis: | Background: Yokukansan (YKS), a traditional herbal (Kampo) medicine consisting of seven herbs, is effective in the treatment of pain disorders, such as headache, postherpetic neuralgia, fibromyalgia, and trigeminal neuralgia, and we have previously shown it to be effective against morphine analgesic tolerance in rats. It has been reported that orexin receptor antagonists prevent the development of morphine tolerance and that YKS inhibits the secretion of orexin A in the hypothalamus. This study examined whether the inhibition of the secretion of orexin A by YKS is one mechanism underlying its effect against morphine analgesic tolerance. Methods: Male Wistar rats were administered a subcutaneous injection of morphine hydrochloride (10 mg/kg/day) for 5 days. One group was preadministered YKS, starting 3 days before the morphine. The withdrawal latency following thermal stimulation was measured daily using a hot plate test. On day 5, the levels of orexin A in the plasma and the midbrain were measured, and the appearance of activated astrocytes in the midbrain was examined by immunofluorescence staining. Results: The preadministration of YKS prevented the development of morphine tolerance. The repeated administration of morphine significantly increased the plasma and midbrain levels of orexin A and the activation of astrocytes. These increases were significantly inhibited by the preadministration of YKS. Conclusion: These results suggest that the preadministration of YKS attenuated the development of antinociceptive morphine tolerance and that the inhibition of orexin A secretion may be one mechanism underlying this phenomenon. Keywords: Astrocyte, Kampo medicine, Morphine tolerance, Orexin A, Yokukansan |
| Databáze: | OpenAIRE |
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