Sex and Depot Differences in Palmitoleic Acid Content of Human Blood and Fat
| Autor: | Michael D. Jensen, Paola Ramos, Nikki C. Bush |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Very low-density lipoprotein Adipose tissue Fatty Acids Nonesterified Biochemistry Article Fatty Acids Monounsaturated Young Adult 03 medical and health sciences chemistry.chemical_compound Absorptiometry Photon Insulin resistance Internal medicine medicine Abdominal fat Humans Palmitoleic acid Obesity Prospective Studies Chromatography High Pressure Liquid Triglycerides Sex Characteristics 030109 nutrition & dietetics Human blood Chemistry Organic Chemistry Cell Biology medicine.disease Lipoproteins LDL 030104 developmental biology Endocrinology Adipose Tissue Female lipids (amino acids peptides and proteins) Insulin Resistance Lipokine Stearoyl-CoA Desaturase Homeostasis |
| Zdroj: | Lipids |
| ISSN: | 1558-9307 0024-4201 |
| Popis: | Palmitoleic acid has been classified as an insulin-sensitizing lipokine, but evidence for this from human studies has been inconsistent. We hypothesized that this is related to either the types of samples or conditions under which samples are collected. We measured plasma palmitoleic acid and total free fatty acids (FFA) using ultra-performance liquid chromatography in blood samples collected from 34 adults under a variety of conditions. We collected duplicate samples of adipose (n = 10), FFA (n = 9), and very low density lipoprotein triacylglycerol (VLDL-TAG) (n = 7) to measure the palmitoleic acid as a percentage of total fatty acids. We tested whether the percentage of palmitoleic acid was correlated with insulin resistance, as measured by homeostatic model of insulin resistance (HOMA-IR). Adipose stearoyl-coenzyme A desaturase 1 (SCD-1) protein was measured by capillary Western blotting. FFA-palmitoleic acid percentage increased as a function of total FFA and was greater (p < 0.005) in females than males. Adipose palmitoleic acid percentage was greater in females than males (p < 0.001), as was adipose SCD-1. Palmitoleic acid was greater in femoral fat than in abdominal fat in both females and males (p < 0.001), and correlated positively with HOMA-IR only in females. The test-retest reliability values for percentage palmitoleic acid were 7 ± 10% for adipose, 24 ± 26% for VLDL, and 53 ± 31% for FFA. Because FFA-palmitoleic acid percentage varies as a function of total FFA, investigators should re-evaluate how palmitoleic acid data is presented. The positive relationship between adipose palmitoleic acid and HOMA-IR in females suggests that it is not a potent insulin-sensitizing lipokine in humans. |
| Databáze: | OpenAIRE |
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