CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes
| Autor: | James M. A. Turner, Obah A. Ojarikre, Shantha K. Mahadevaiah, Charlotte Douglas, Daniel M Snell, Valdone Maciulyte, Peter J. I. Ellis, Jasmin Zohren |
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| Rok vydání: | 2021 |
| Předmět: |
Model organisms
Litter (animal) Male Single sex CRISPR-Cas systems Litter Size Science Gene Expression General Physics and Astronomy Mice Transgenic Culling Biology General Biochemistry Genetics and Molecular Biology Article Mice Pregnancy Developmental biology Genetics CRISPR Animals SF QP506 Animal Husbandry Laboratory research QH426 QH581.2 Gene Editing Human Biology & Physiology QL Multidisciplinary Effector Stem Cells Genome Integrity & Repair Cell Biology General Chemistry Sex Determination Processes Phenotype Sex specific Evolutionary biology Genetic engineering Models Animal Cell Cycle & Chromosomes Female Synthetic Lethal Mutations Genetics & Genomics Selective Breeding |
| Zdroj: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-021-27227-2 |
| Popis: | Animals are essential genetic tools in scientific research and global resources in agriculture. In both arenas, a single sex is often required in surplus. The ethical and financial burden of producing and culling animals of the undesired sex is considerable. Using the mouse as a model, we develop a synthetic lethal, bicomponent CRISPR-Cas9 strategy that produces male- or female-only litters with one hundred percent efficiency. Strikingly, we observe a degree of litter size compensation relative to control matings, indicating that our system has the potential to increase the yield of the desired sex in comparison to standard breeding designs. The bicomponent system can also be repurposed to generate postnatal sex-specific phenotypes. Our approach, harnessing the technological applications of CRISPR-Cas9, may be applicable to other vertebrate species, and provides strides towards ethical improvements for laboratory research and agriculture. In areas such as animal research and agriculture a single sex is often required in abundance, leading to wasted resources and ethical considerations. Here the authors develop a CRISPR/Cas9 mediated synthetic lethal system that enables the production of single sex offspring that can be repurposed for use in multiple organisms. |
| Databáze: | OpenAIRE |
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