Hemoglobin–albumin cluster: physiological responses after exchange transfusion into rats and blood circulation persistence in dogs
| Autor: | Hitomi Iwasaki, Kyoko Yokomaku, Ryosuke Funaki, Ryo Hashimoto, Keisuke Igarashi, Motofusa Akiyama, Moeka Kureishi, Risa Haruki, Kenichi Asai, Mitsutomo Kohno, Masayuki Iwazaki, Yuka Nakamura, Yoshitsugu Morita, Teruyuki Komatsu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Exchange Transfusion Whole Blood Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Exchange transfusion Serum Albumin Human 02 engineering and technology Pharmacology Beagle Blood substitute Blood cell Hemoglobins 03 medical and health sciences Dogs medicine Animals Whole blood Hemodilution business.industry Albumin General Medicine 021001 nanoscience & nanotechnology Rats body regions Red blood cell 030104 developmental biology medicine.anatomical_structure embryonic structures Hemoglobin 0210 nano-technology business Half-Life Biotechnology |
| DOI: | 10.6084/m9.figshare.7518902 |
| Popis: | A core–shell protein cluster comprising hemoglobin and human serum albumins, hemoglobin–albumin cluster (Hb–HSA3), was designed and synthesized for use as an artificial O2 carrier and red blood cell (RBC) substitute. For initial preclinical safety evaluation of the Hb–HSA3 solution, we observed blood compatibility in vitro, physiological responses after exchange transfusion into rats and blood circulation lifetime in dogs. Dilution of human whole blood with Hb–HSA3 showed an appropriate decrease in blood cell number, proportional to the mixing volume ratio. Time courses in the circulation parameters and blood gas parameters after 20% exchange transfusion with Hb–HSA3 in anesthetized rats were almost identical to those observed in a sham group (without infusion) and an HSA group (with HSA administration) for 6 h. Serum biochemical tests of the withdrawn blood indicated safety of the protein cluster. Furthermore, fluorescent Hb–HSA3 was infused into beagle dogs to assess blood retention. Fluorescence measurements of the blood samples enabled us to ascertain the cluster half-life within the intravascular space. Histopathologic inspections of the vital organs imply no abnormality in tissues. All these results indicate sufficient initial preclinical safety of Hb–HSA3 as an alternative material for use in RBC transfusion. |
| Databáze: | OpenAIRE |
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