High throughput characterization of structural differences between closely related proteins in solution
| Autor: | Vladimir N. Uversky, Boris Y. Zaslavsky, Pedro P. Madeira, Leonid Breydo, Alexander Zaslavsky, Arnon Chait |
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| Rok vydání: | 2013 |
| Předmět: |
Aqueous solution
Chromatography Aqueous medium Swine Chemistry Biophysics Aqueous two-phase system Proteins A protein Biochemistry Protein Structure Tertiary Analytical Chemistry Characterization (materials science) Structure-Activity Relationship Protein structure Animals Humans Cattle Biological system Nuclear Magnetic Resonance Biomolecular Molecular Biology Throughput (business) |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1834:583-592 |
| ISSN: | 1570-9639 |
| DOI: | 10.1016/j.bbapap.2012.11.004 |
| Popis: | Partitioning of a protein in an aqueous two-phase system (ATPS) is governed by interactions of the protein with aqueous media in the two phases. Here we describe how partitioning of proteins in a set of ATPS of different compositions can be used to quantify differences between 3D structures of closely related proteins. We also provide perspective on practical applications of the technology when comparative analysis of the higher-order structure of proteins is desired. |
| Databáze: | OpenAIRE |
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